用组织培养、细胞悬浮培养和单细胞平板培养技术, 诱导出冬凌草愈伤组织, 并探讨了细胞悬浮培养时间、培养方法和接种密度对冬凌草单细胞平板培养植板率的影响.结果表明:从冬凌草叶和嫩茎诱导愈伤组织, 以MS+2, 4-D1mg/L+NAA0.5mg/L培养基较好, 愈伤组织诱导率高达96.80%.用普通单细胞平板培养法培养冬凌草单细胞的植板率很低, 而以悬浮培养15～18d的单细胞为材料, 接种密度为5×103个/毫升时, 进行条件培养和看护培养, 植板率达21.63%.本研究结果可供利用细胞培养技术筛选冬凌草高产冬凌草素细胞株时参考.

Conditions for cell culture of Rabdosia rubes cens (Hemsl) Hara. were studied with the aim to develop a new source of ru bescensin for antitumor therapy. Calli were induced from the leaf and stem of R. rubes cens and cultured either by cell suspension culture or unicellular plate c ulture. Fac tors affecting plate efficiency, such as cell suspension culture time, ways of p la ting and cell density were studied. Culture medium MS containing 1∶0.5 ratio of 2, 4D∶NAA was found to be the most suitable medium for the induction of calli which attained an induction rate well over 96.8%. High pla ting efficiency could be obtained by plating at a density of 5×103 cells/mL w ith monocells separated from 15.18 d cells.Result of the present study may pro vide some reference for the screening of high yield cell strain for the producti on of rubescensin.