目的:研究鲤鱼精巢DNA对自由基的清除作用.方法:采用化学发光法测定鲤鱼精巢DNA对Cu²⁺-Vit C-H₂O₂发光体系产生的·OH自由基的清除作用, 采用分光光度法检测鲤鱼精巢DNA对大鼠腹腔多形核白细胞(PMNs)呼吸爆发产生O₂^-自由基的清除作用, 采用电子自旋共振(ESR)法测定鲤鱼精巢DNA对DPPH有机自由基的清除作用.结果:鲤鱼精巢DNA使Cu²⁺-Vit C-H₂O₂发光体系的发光计数率降低, 使PMNs呼吸爆发产生O₂^-自由基的数量减少, 使DPPH的特征波峰逐渐降低直至消失.结论:鲤鱼精巢DNA对·OH自由基、O₂^-自由基和有机自由基均有显著的消除作用.

To investigate whether DNA from carp spermary scave nges free radicals. Chemical luminometry, spectrometry and electronic spin resonanc e (ESR) methods were used to measure the scavenging action of deoxyribonucleic ac id (DNA) from carp spermary on ·OH radical produced by Cu²⁺-Vit C-H₂O₂ chemoluminescent system, O₂^- radical produced by the breathing burst of polymorphonuclear leukocytes (PMNs) and DPPH radical respectively. DNA from car p spermary scavenged ·OH radical produced by Cu²⁺-Vit C-H₂O₂ chemolu minescent system, O₂^- radical produced by the breathing burst of PMNs and DPPH radicals. It could be concluded that DNA from carp spermary scavenges free radicals.