采用聚丙烯酰胺凝胶电泳活性染色法,观察复方生脉饮对小鼠心肌乳酸脱氢酶(LH)同工酶的影响。结果表明:a)服用复方生脉饮10d的小鼠LDH总比活力比对照组提高13%。b)实验组小鼠心肌LDH同工酶谱发生显著变化,LDH₁和LDH₂增加,LDH₄与LDH₃减少。c)与对照组比较,实验组小鼠心肌LDH1-2比活力提高38%,LDH_4-5比活力降低31%。d)小鼠剧烈运动后血乳酸清除速率显著加快。结果提示复方生脉饮改变LDH同工酶谱,增加LDH_1-2此活力是其一个重要的抗疲劳机制。

Effects of"Pulse Vitalizing Tonic Compound"(Fufangshengmaiyin,FSMY)on mouse heart lactate dehy-drogenase (LDH)isozyme was examined with polyacrylamide gel electrophoresis and active staining. Results showed that:(1) Total specific activity of LDH was increased by 1300 after taken FSMY for 10 days,as com-pared with the control. (2)LDH isozyme map of heart muscle changed significantly with increase of LDH₁ and LDH₂ and decreased of LDH₄ and LDH₅. (3) Specific activities of LDH_1-2 was elevated by 3800,while that of LDH_4-5:lowered by 31%as compared with the control. (4)Blood lactic acid clearance was accelerated after vig-orous excercices. The above results pointed out that influence on LDH isozyme map and LDH_1-2 specific activi-ties are important aspects of anti-fatigue mechanism of FSMY.