用电泳方法分析了浙贝母低温解除休眠过程中芽、鳞片近轴面表皮和鳞片内部组织的酯酶同工酶和可溶性蛋白质的变化。芽的酯酶同工酶谱带较多, 变化明显。鳞片内部组织的酯酶同工酶谱带较少,变化较小。芽、鳞片近轴面表皮和鳞片内部组织可溶性蛋白质图谱的变化主要是颜色深浅的变化。结果说明浙贝母低温解除休眠的过程是由芽和鳞片共同完成的, 鳞片近轴面表皮在芽和鳞片内部组织的相互关系中起着重要作用。

Changes of esterase iaozyme and soluble protein in bud, adaxial cortex and inner tissue in scale were assayed by electrophoresis over the course of dormancy relieving in Fritillaria thunbergii. Esterase isozyme pattern of bud had more hands than the others, and these bands changed significantly as compared to the adaxial cortex and inner tissue in scale. The bands in esterase isozyme pattern of inner tissue in scale were few and changed little than the others. Changes of soluble protein patterns in bud, adaxial cortex and inner issue in scale were mainly on color. These results indicated that both the bud and the scale joined the course of dormancy relieving of Fritillaria thunbergii, and the adaxial cortex presented role in the relation between the bud and the inner issue in scale.