胡琦,朱定耀,谭小宁,李勇敏,朱克俭.臭牡丹提取物对H22荷瘤小鼠体内抑瘤作用及对相关蛋白表达的影响[J].药物评价研究,2019,42(7):1309-1313
臭牡丹提取物对H22荷瘤小鼠体内抑瘤作用及对相关蛋白表达的影响
Anti-tumor effect of Clerodendron bungei extract on H22 tumor-bearing mice and its effect on expression of related proteins
投稿时间:2019-03-07  
DOI:10.7501/j.issn.1674-6376.2019.07.009
中文关键词:  臭牡丹;H22荷瘤小鼠;Bcl-2;Bax
英文关键词:Clerodendron bungei;H22 tumor-bearing mice;Bax;Bcl-2
基金项目:国家中医药管理重大项目(国中医药人教函[2012]228号);湖湘欧阳氏杂病流派辩证恶性肿瘤学术经验的研究(2017XJJJ21)
作者单位E-mail
胡琦 湖南中医药大学, 湖南 长沙 410208  
朱定耀 湖南中医药大学, 湖南 长沙 410208  
谭小宁 湖南省中医药研究院附属医院, 湖南 长沙 410006  
李勇敏 湖南省中医药研究院附属医院, 湖南 长沙 410006  
朱克俭 湖南省中医药研究院附属医院, 湖南 长沙 410006 zkjo0731@263.net 
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中文摘要:
      目的 研究臭牡丹提取物对H22实体移植瘤的抑制作用及与B淋巴细胞瘤/白血病-2(Bcl-2)和Bcl-2相关X蛋白(Bax)表达的相关性。方法 皮下接种H22瘤株建立小鼠H22实体移植瘤模型。60只荷瘤鼠随机分为模型组、5-氟尿嘧啶(5-Fu)组和臭牡丹高、中、低剂量(2.18、1.09、0.55 g/kg)组,每组12只,各组分别给予生理盐水、5-Fu(25 mg/kg,1次/d)及不同浓度的臭牡丹提取物干预。连续给药14 d后处死,检测小鼠体质量变化、抑瘤率及脾脏和胸腺指数;Western blot法检测Bcl-2和Bax蛋白的表达水平。结果 与模型组比较,臭牡丹高、中剂量组小鼠的肿瘤质量均显著降低(P<0.01),胸腺和脾脏指数显著增加(P<0.05);同时Bax蛋白的表达水平显著上调,Bcl-2蛋白的表达显著降低(P<0.01)。结论 臭牡丹提取物能够抑制H22实体移植瘤生长,其机制可能与上调Bax/Bcl-2比值和免疫调节有关。
英文摘要:
      Objective To study the inhibitory effect of Clerodendron bungei extract on H22 solid xenograft and its correlation with the expression of B cell lymphoma/leukemia-2 (Bcl-2) and Bcl-2 related X protein (Bax) proteins. Methods A mouse H22 solid xenograft model was established by subcutaneous inoculation of H22 tumor strain. Sixty tumor-bearing mice were randomly divided into model group, 5-fluorouracil (5-Fu) group and Clerodendron bungei high, medium and low dose groups, 12 rats in each group. Each group was given normal saline and 5-Fu (25 mg/kg, 1 time/d) and different concentrations of Clerodendron bungei intervention. After 14 days of continuous administration, the tumor was sacrificed, and the changes in body mass, tumor inhibition rate and spleen and thymus index were measured. Western blot was used to detect the expression levels of Bcl-2 and Bax proteins. Results Compared with the model group, the tumor mass of the high, middle and low dose groups of the Clerodendron Bungei group was significantly decreased (P<0.01), and the thymus and spleen index increased (P<0.05). At the same time, the expression level of bax protein was up-regulated and the expression of bcl-2 protein was decreased (P<0.01).Conclusion The Clerodendron bungei extract can inhibit the growth of H22 solid xenografts, and its mechanism may be related to up-regulating the ratio of bax/bcl- 2 to induce apoptosis.
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