目的 研究注射用益气复脉（冻干）（YQFM）对大鼠离体胸主动脉环的作用及机制。方法 采用大鼠离体胸主动脉血管环，观察YQFM及红参、麦冬、五味子提取物（HS、MD、WWZ）对血管的直接作用，YQFM对KCl或去甲肾上腺素所引发血管收缩作用的影响；对乙酰胆碱所致血管舒张作用的影响；对内钙释放所致血管收缩作用的影响。结果 YQFM在低浓度下对血管无刺激作用，但是在高浓度时，对血管有刺激收缩作用，且无内皮组收缩幅度明显超出有内皮组。不同浓度的3种药材提取物对血管刺激作用不显著；YQFM可显著抑制KCl或者去甲肾上腺素引起的血管收缩；YQFM对乙酰胆碱引起的血管舒张作用无显著抑制作用；YQFM不能抑制内钙释放。结论 YQFM在高浓度下对血管有一定的直接刺激收缩作用，且直接刺激作用可能和血管内皮相关；YQFM既有抑制受体操纵性钙通道又有抑制电压依赖性钙通道的调节作用。

Objective This study explored the effect and mechanism of Yiqi Fumai Lyophilized Injection (YQFM) on the isolated thoracic aortic rings in rats.Methods The isolated thoracic aorta rings of SD rats were used to observe:the direct effects of YQFM and hongshen extract, maidong extract, wuweizi extact on the vascular rings, effect of YQFM on vasoconstriction induced by potassium chloride or phenylephrine, effect on vasodilatation induced by acetylcholine, effect of endocalcium release on vasoconstriction.Results At low concentrations, YQFM has no stimulating effect on thoracic aortic rings. At high concentrations, YQFM has a stimulating contraction effect on blood vessels and the amplitude of contraction in the non-endothelial group is significantly higher than that in the endothelial group; the effects of the three extracts on thoracic aortic rings were weak, at high concentration, the effect has no significant difference; the vasoconstriction effect induced by potassium chloride or phenylephrine was inhibited by YQFM; the vasodilatation effect induced by acetylcholine was not inhibited. YQFM could not inhibit the release of internal calcium.Conclusions At high concentration, YQFM has a certain direct stimulation and contraction effect on blood vessels, and the stimulation effect may be related to multiple components. Moreover, the vascular endothelium may be the potential target of the direct stimulation effect. Both the receptor-manipulative calcium channel and the voltage-dependent calcium channel can be inhibited by YQFM.

天津市中药注射剂关键技术校企协同创新实验室建设项目（17PTSYJC00090）