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[摘要]
目的 考察玄归滴丸对乙醇致大鼠急性胃溃疡的保护作用并探讨其作用机制。方法 65只SD大鼠随机分为对照组、模型组、西咪替丁(50 mg/kg)组和玄归滴丸低、中、高剂量(50、100、200 mg/kg,分别为临床等效剂量的2、4、8倍)组。给药组ig给予相应的受试药物,对照组及模型组ig相同剂量的生理盐水,连续7 d,除了对照组外,其余各组均ig无水乙醇制备乙醇型胃溃疡模型。造模1 h后处死大鼠,取胃组织进行大体形态观察,并进行HE染色,光镜下观察病理形态变化;试剂盒法检测胃组织中超氧化物歧化酶(SOD)、丙二醛(MDA)、髓过氧化物酶(MPO)、肿瘤坏死因子-α(TNF-α)、前列腺素E2(PGE2)和解痉多肽(SP)含量。结果 玄归滴丸组的胃黏膜损伤程度、病理变化与模型组比较均有不同程度的减轻。与模型组比较,玄归滴丸中剂量组SOD和低、中剂量组MPO水平均显著升高(P<0.05、0.01);中剂量组MDA水平显著降低(P<0.05);各剂量组TNF-α水平呈降低趋势;低剂量组PGE2含量显著升高(P<0.05);高剂量组SP含量显著降低(P<0.05)。结论 玄归滴丸对乙醇致急性胃溃疡实验大鼠的胃黏膜损伤有较好的保护作用,其作用机制可能与抗氧化、抗炎作用有关。
[Key word]
[Abstract]
Objective To investigate the protective effect of Xuangui Dropping Pills on alcohol-induced acute gastric ulcer in rats and explore its mechanism.Methods A total of 65 SD rats were randomly divided into control group, model group, cimetidine group (50 mg/kg) and Xuangui Dropping Pills low, medium and high dose (50, 100 and 200 mg/kg, 2, 4, 8 times of clinical equivalent dose, respectively) groups. The rats in the treatment group were given the corresponding drugs by gavage. The rats in control group and model group were fed with the same dose of saline for seven consecutive days. Except for control group, modeling of experimental gastric ulcer was carried out in all the other groups with anhydrous ethanol. Rats were executed one hour after establishment of model, and the gastric tissues were taken for gross morphological observation, HE staining and light microscopy were used to observe the pathological changes. The contents of superoxide dismutase (SOD), malondialdehyde (MDA), myeloperoxidase (MPO), tumor necrosis factor-alpha (TNF-alpha), prostaglandin E2 (PGE2) and spasmolytic polypeptide (SP) in gastric tissues were detected by kit method. Results Compared with model group, the degree of gastric mucosal injury and pathological changes in Xuangui Dropping Pills group were alleviated in varying degrees. Compared with model group, the SOD and MPO levels of Xuangui Dropping Pills in middle dose group increased significantly (P<0.05 and 0.01), while the MDA level in middle dose group decreased significantly (P<0.05). Compared with control group, the level of TNF-alpha in model group increased, and the content of TNF-α decreased after Xuangui Dropping Pill intervention. Compared with model group, the content of PGE2 in Xuangui Dropping Pills low dose group was significantly higher (P<0.05), while the content of SP in high dose group was significantly lower (P<0.05). Conclusion Xuangui Dropping Pills can protect gastric mucosal injury induced by ethanol in rats with acute gastric ulcer. Its mechanism may be related to the regulation of SOD, MPO, SP, TNF-α and PGE2 expression.
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[基金项目]
长江学者和创新团队发展计划资助项目(IRT_14R41)