蔡曼曼1,龙子江1 *,周宜轩2,丁伯春1,陈 铖1,陆松侠1.养肝益水颗粒对自发性高血压大鼠肾组织TGF-β1、PI3K和PKB的影响[J].药物评价研究,2013,36():1-4
养肝益水颗粒对自发性高血压大鼠肾组织TGF-β1、PI3K和PKB的影响
Effects of Yanggan Yishui Granule on TGF-β1, PI3K and PKB expression in renal tissue of spontaneously hypertensive rats
投稿时间:2013-04-15  
DOI:
中文关键词:  养肝益水颗粒;自发性高血压大鼠;转化生长因子-β1;磷脂酰肌醇-3激酶;蛋白激酶B;尿微量白蛋白
英文关键词:Yanggan Yishui granule; SHR; TGF-β1; PI3K; PKB; UMA
基金项目:康缘中医药科技创新基金项目(2010HZ020)
作者单位
蔡曼曼1,龙子江1 *,周宜轩2,丁伯春1,陈 铖1,陆松侠1 1. 安徽中医药大学,安徽 合肥 230038;2. 安徽中医药大学第一附属医院,安徽 合肥 230031 
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中文摘要:
      目的 观察养肝益水颗粒对自发性高血压大鼠(spontaneously hypertensive rat,SHR)肾组织转化生长因子-β1(TGF-β1)、磷脂酰肌醇-3激酶(PI3K)和蛋白激酶B(PKB)的影响,探讨养肝益水颗粒对原发性高血压早期肾损害的作用及作用机制。方法 取50只12周龄收缩压大于150 mmHg的SHR,随机分为模型组,养肝益水颗粒高、中、低剂量组(10.8、5.4、2.7 g/kg)和阳性对照药厄贝沙坦组(0.015 g/kg),每组10只;另取10只Wistar雄性大鼠作为正常对照组,模型组和正常对照组ig给予等容量蒸馏水,每天1次,共12周。给药前和给药12周后用酶联免疫吸附法(ELISA)检测各组大鼠尿微量白蛋白(UMA)浓度;末次给药后,用免疫组化法检测各组大鼠肾脏组织中TGF-β1和PI3K的表达;用ELISA法检测PKB的浓度。结果 养肝益水颗粒有效的降低了尿微量白蛋白的浓度和肾脏组织TGF-β1;升高了肾脏组织PI3K和PKB水平。结论 养肝益水颗粒对高血压的早期肾损害有一定的抑制作用,其作用机制可能与影响TGF-β1、PI3K和PKB的含量有关
英文摘要:
      Objective To observe the effects of Yanggan Yishui granule (YGYSG) on Transforming Growth Factor-β1 (TGF-β1), PhosPhatidylinositol-3-kinase (PI3K) and protien kinase B (PKB) in spontaneously hypertensive rats (SHR), in order to explore the underlying mechanisms of YGYSG in preventing and treating early renal damage. Methods Fifty 12 weeks old male and the systolic blood pressure > 150 mmHg SHR were randomly divided into the model group (the same volume of distilled water), the YGYSG high, medium and low dose group (at the daily dose of 10.8 g /kg, 5.4 g /kg and 2.7 g /kg YGYSG), and the positive control group (at the daily dose of 15 mg/kg irbesartan), 10 in each group. Another ten Wistar rats were included as the control group (the same volume of distilled water). The drug was given by intragastric gavage for 12 weeks, respectively, at pre-administration and after successive administration for 12 weeks, urine Microalbumin (urine MA) concentration was measured by ELISA; after the last administration, the levels of TGF-β1 and PI3K in liver tissue were determined by immunohistochemistry and PKB were detected by ELISA. Results YGYSG decreased urine MA and TGF-β1 concentration, and increased PI3K and PKB concentration. Conclusion Yanggan Yishui granule inhibited early renal damage of SHR which might be related to its regulation on TGF-β1, PI3K and PKB concentration
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