目的 评价注射用丹参多酚酸（SAFI）对血小板功能的影响。方法 采用大鼠体内实验和家兔体外实验。在体内实验中，40只大脑缺血-再灌注模型（MCAO/IR）大鼠随机分为模型组，SAFI低、中、高剂量（5.8、11.6、23.2 mg/kg）组和阳性药（银杏内酯注射液，2 mL/kg）组，另取8只切开颈部皮肤作为假手术组。尾静脉iv给药14 d后分别取血，凝血时间检测试剂盒检测活化凝血时间（ACT）和活化部分凝血活酶时间（APTT），利用ELISA试剂盒测定各组血清中5-羟色胺（5-HT）、P选择素（CD62P）、β球蛋白（β-TG）和血小板膜糖蛋白Ⅱa/Ⅲb（GPⅡa/Ⅲb）含量。体外实验中，采用腺苷二磷酸（ADP），血小板活化因子（PAF）和花生四烯酸（AA）为诱导剂，诱导家兔血小板聚集，通过血小板分析仪测定不同浓度的SAFI对血小板聚集率的影响。结果 体内实验结果表明：低、中、高剂量SAFI均能明显延长大鼠血液凝血时间，且能有效降低模型大鼠血液中5-HT、CD62p、β-TG和GPⅡb/Ⅲa的水平（P<0.05、0.01）。体外实验结果表明：SAFI均可显著降低血小板聚集率、抑制血小板聚集（P<0.05、0.01）。结论 SAFI可有效抑制血小板活化，改善血小板的黏附、聚集、释放等功能。

Objectives To explore the effect of Salvianolic Acids for Injection (SAFI) on platelet function. Methods The effects were evaluateed by rats experiment in vivo and rabbits experiment in vitro. In vivo experiments, cerebral ischemia-reperfusion model (MCAO/IR) rats were constructed by carotid artery embolization, and 40 rats that were successfully modeled were randomly divided into model group, SAFI low-dose group (5.8 mg/kg), medium-dose group (11.6 mg/kg) and high-dose group (23.2 mg/kg), and positive drug group (Ginkgolides Injection, 2 mL/kg). Another eight rats were cut into the neck skin without modeling as the sham operation group. Blood samples were collected after 14 days of caudal vein administration. Activated coagulation time (ACT) and activated partial thrombin time (APTT) were measured using a clotting time detection kit. The contents of 5-HT, CD62P, β-TG and GP Ⅱ a/Ⅲ b in serum of each group were determined by ELISA kit. In vitro experiment, adenosine diphosphate (ADP), platelet activating factor (PAF) and arachidonic acid (AA) were used as inducers to induce platelet aggregation in rabbits. Results The results of in vivo experiments showed that low, medium and high dose SAFI can significantly prolong the blood coagulation time of rats, and effectively reduced the levels of 5-HT, CD62p, β-TG and GPⅡa/Ⅲb in blood of model rats (P<0.05, 0.01). The results of in vitro experiments showed that SAFI could significantly reduce the platelet aggregation rate and inhibit platelet aggregation in ADP, PAF and AA induced platelet aggregation (P<0.05, 0.01). Conclusions SAFI can effectively inhibit platelet activation and improve platelet adhesion, aggregation and release.

R285.5