[关键词]
[摘要]
目的 以斑马鱼为模型,评价三峡阳菊叶水提物(WELTGY)对斑马鱼整体形态以及心脏、肾脏和肝脏等主要脏器的影响。方法 用不同浓度(2.8、3.4、3.8、4.8、5.8、6.8 mg/mL)的WELTGY处理受精后72 h(72 hpf)的斑马鱼,处理24、48 h后,观察斑马鱼死亡率、畸形率和整体形态;显微镜下观察斑马鱼心脏形态并拍照,记录心率,使用ImagePro Plus 5.1测量斑马鱼静脉窦-动脉球(SV-BA)距离,评价WELTGY对斑马鱼心脏的影响;显微镜下观察拍照记录各组斑马鱼是否有眼水肿,判断WELTGY对斑马鱼肾脏的影响;用WELTGY处理肝脏标记绿色荧光的转基因斑马鱼Tg(LFABP:EGFP),荧光显微镜下拍照记录斑马鱼肝脏荧光情况,评价WELTGY对斑马鱼肝脏的影响。结果 与对照组比较,2.8 mg/mL及以下浓度的WELTGY对斑马鱼的整体形态、心脏、肾脏和肝脏无明显影响;3.4 mg/mL以上浓度的WELTGY导致斑马鱼鱼鳔减小,对心脏、肝脏、肾脏无影响;4.8 mg/mL以上浓度的WELTGY导致斑马鱼心率显著降低(P<0.01),对心脏SV-BA距离、肾脏、肝脏荧光强度和荧光面积无明显影响。结论 WELTGY毒性微弱,对斑马鱼幼鱼几乎没有毒性;高浓度下会导致轻微心脏毒性,在家禽养殖业的使用中有必要合理控制用量。
[Key word]
[Abstract]
Objective To evaluate the effects of water extract from leaves of Three Gorges Yangju (WELTGY) on the development of morphology and main organs such as heart, kidney and liver by zebrafish model. Methods Zebrafish embryos at 72 hpf (hours post fertilization) were treated with different concentrations (2.8, 3.4, 3.8, 4.8, 5.8, and 6.8 mg/mL) of WELTGY. The mortality rate, malformation rate and overall morphology of zebrafish were observed after treatment for 24 and 48 h. The heart morphology of zebrafish was observed and photographed under a microscope, and the heart rate was recorded. Image-Pro Plus 5.1 was used to measure the distance between the venous sinuso-artery bulb (SV-BA) of zebrafish, so the effect of WELTGY on the heart of zebrafish was evaluated. The effect of WELTGY on the kidney of zebrafish was determined by observing and photographing zebrafish eye edema in each group under microscope. The liver labeled green fluorescent transgenic zebrafish Tg(L-FABP:EGFP) was treated with WELTGY, and the liver fluorescence of zebrafish was photographed under fluorescence microscope to evaluate the effect of WELTGY on the liver of zebrafish. Results WELTGY at a concentration of 2.8 mg/mL or less had no effect on the overall morphology, heart, liver and kidney of zebrafish. Concentrations above 3.4 mg/mL caused the swim bladder to decrease, and had no effects on the heart, kidney, and liver. Concentrations above 4.8 mg/mL caused a significant decrease in zebrafish heart rate, and had no effects on the distance between the heart venous sinus -bulus arteriosus, kidney, liver fluorescence intensity, and fluorescence area. Conclusion In this study, WELTGY was found to be weakly toxic and had almost no effects on zebrafish embryogenesis. High concentrations could cause slight cardiotoxicity. It is necessary to strengthen the management and control the dosage in the use of poultry breeding.
[中图分类号]
R965.3
[基金项目]
山东省技术创新引导计划(鲁渝科技协作)项目(2020LYXZ017);重庆市科技局鲁渝科技合作项目(CSTC2020JSCX-LYJ‐SA0030);山东省优秀青年人才基金项目(ZR2020YQ60);齐鲁工业大学(山东省科学院)科教产融合创新试点工程项目(2020KJC-ZD08)