[关键词]
[摘要]
目的 建立临床前毒理学研究中Beagle犬中枢神经系统一致性制片方法。方法 以Beagle犬脑组织肉眼可见的解剖部位,如额极、视交叉、动眼神经、脑桥、前庭耳蜗神经为标志,对Beagle犬脑组织(腹侧面向上)进行冠状水平单侧取材。并且对Beagle犬脊髓的颅颈段(C1~C2)、胸中段(T6~T8)和腰膨大处(L4~L5)进行横切面及倾斜横向面取材,并经常规脱水、包埋、切片、HE染色处理。结果 通过这种方法制作的Beagle犬中枢神经系统组织学切片一致性高、质量好,包括了Beagle犬中枢神经系统主要结构,例如尾状核、基底核、大脑皮质(额叶、顶叶、枕叶、颞叶)、脉络丛、海马、下丘脑、延髓、中脑、脑桥、丘脑、小脑和脊髓。结论 该制片方法简单易行且一致性高,可用于临床前毒理学研究中Beagle犬中枢神经系统组织病理学检查的一致性制片。
[Key word]
[Abstract]
Objective To establish a consistent slide preparation method of Beagle dog central nervous system (CNS) for preclinical toxicology studies. Methods The anatomical parts visible outside the brain tissue, such as the frontal pole, optic chiasm, oculomotor nerve, pons, and vestibular cochlear nerve were used as landmarks. Unilateral sampling was performed at the coronal horizontal level of the brain tissue of Beagle dog (ventral side up). Moreover, cross sections and oblique transverse sections were obtained from the cranio cervical segments of spinal cord (C1-C2), middle thoracic segment (T6-T8) and lumbar enlargement (L4-L5) of Beagle dog. The samples were routinely dehydrated, embedded, sectioned and HE stained. Results The histological sections of the CNS of Beagle dog were produced with high quality and good consistency and contained major structures of CNS, such as the caudate nucleus, basal ganglia, cerebral cortex (frontal lobe, parietal lobe, occipital lobe and temporal lobe), choroid plexus, hippocampus, hypothalamus, medulla oblongata, midbrain, pons, thalamus, cerebellum and spinal cord. Conclusion The method is easy and consistent and can be used for preparation of the consistent slides for histopathological examination of the CNS of Beagle dog in preclinical toxicology studies.
[中图分类号]
[基金项目]
国家"重大新药创制"科技重大专项课题(2018ZX09201-017)