[关键词]
[摘要]
目的 研究淫羊藿水提后药渣对去势雄性大鼠的壮骨作用。方法 雄性SD大鼠去睾丸造成激素缺乏型骨质疏松模型,造模12周后,连续ig给予淫羊藿水提物及药渣8周,给药浓度为0.5、1.0 g/kg(以生药计)。给药结束后处死动物并进行血清生化和骨微结构检测。结果 给药8周,淫羊藿药渣0.5 g/kg组血清I型前胶原N端前肽(P1NP)含量较模型组明显升高(P<0.05),发挥促进骨形成作用;血清尿素氮(BUN)、肌酐(Crea)、N-乙酰-β-D-葡萄糖苷酶(NAG)、天冬氨酸转氨酶(AST)、丙氨酸转氨酶(ALT)、总胆固醇(TC)、三酰甘油(TG)、总蛋白(TP)、白蛋白(ALB)、血糖(GLU)水平均呈降低趋势,肝肾毒性不明显;CT扫描结果显示,与模型组比较,淫羊藿药渣0.5 g/kg组胫骨直径显著升高(P<0.05);水提物0.5 g/kg、药渣1.0 g/kg组骨总体积(TV)显著升高(P<0.05),各给药组骨小梁的体积(BV)均呈升高趋势,骨表面积(BS)、骨小梁数量(Tb.N)均显著升高(P<0.05、0.01);药渣1.0 g/kg组骨小梁分离度(Tb.Sp)显著降低(P<0.01)。结论 0.5和1.0 g/kg淫羊藿药渣对去势诱导的雄性大鼠骨质疏松有治疗作用,效果不低于水提物且没有显著毒性。
[Key word]
[Abstract]
Objective To study toxicity and therapeutic effect of the rest of epimedium aqueous extract on osteoporosis in orchidectomized male rats. Methods Male SD rats were orchidectomized to establish osteoporosis model. After 12 weeks of modeling, the aqueous extract of epimedium and the residue were given by ig for 8 weeks continuously, and the concentration was 0.5 and 1.0 g/kg. Then, animals were sacrificed and performed serum biochemical and bone microstructural tests. Results After 8 weeks of administration, the level of serum I type procollagen N terminal propeptide (P1NP) in 0.5 g/kg epimedium residue group was significantly higher than that of model group (P < 0.05), which played an important role in promoting bone formation. The biochemical results showed that the levels of serum urea nitrogen (BUN), creatinine (Crea), N-acetyl-beta-D-glucosamidase (NAG), aspartate aminotransferase (AST), alanine aminotransferase (ALT), total cholesterol (TC), triacylglycerols (TG), total protein (TP), albumin (ALB), and blood glucose (GLU) were in a decreasing trend and the toxicity of liver and kidney was not obvious. Compared with the model group, CT scanning results showed that the diameter of tibia increased significantly in 0.5 g/kg epimedium residue group (P<0.05); Total volumne of 0.5 g/kg aqueous extract and 1.0 g/kg epimedium residue group was increased significantly (P<0.05); In each group, the trabecular bone volume (BV), bone surface (BS) area and trabecular number (Tb.N) were significantly increased (P<0.05, 0.01); The separation degree of trabecular bone (Tb.Sp) was significantly decreased in 1.0 g/kg epimedium residue group (P<0.01). Conclusion The epimedium residue (0.5 and 1 g/kg) have a significant therapeutic effect on osteoporosis male rats, the effect has no significant difference from aqueous extract of epimedium, and there are no significant toxicity.
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[基金项目]
国家重大新药创制专项课题(2011ZX09201-201-21;2014ZX09304307-001-005)