[关键词]
[摘要]
目的 建立小鼠1型糖尿病模型,研究糖尿病早期时睾丸中睾酮合成关键酶的变化。方法 雄性成年鼠20只,随机分为对照组10只、糖尿病模型组10只。通过ip链脲佐菌素(150 mg/kg)制备糖尿病模型,成功制备糖尿病模型后4周处死小鼠,取血清和睾丸组织备用。实时荧光定量PCR(qRT-PCR)法检测睾丸组织中促黄体生成素受体(LHR)、类固醇激素合成灵敏调节蛋白(StAR)、细胞色素P450胆固醇侧链裂解酶(P450scc)、3β-羟基类固醇脱氢酶VI型(3β-HSD6)、17a-羟基化酶/17,20-裂解酶I型(P450c17a1)及17β-羟基类固醇脱氢酶Ⅲ型(17β-HSD3)相应mRNA(Lhcgr、Star、Cyp11a1、Hsd3b6、Cyp17a1和Hsd17b3)含量,酶联免疫吸附法测定血清中睾酮和黄体生成素(LH)含量、睾丸组织中3β-HSD1、P450c17a1及17β-HSD3酶活性。结果 与对照组比较,4周糖尿病小鼠血清中睾酮和LH含量显著降低(P<0.05);睾丸组织的Lhcgr、Star、Cyp11a1、Hsd3b6、Cyp17a1和Hsd17b3 mRNA含量显著降低(P<0.05、0.001);3β-HSD1、P450c17及17β-HSD3酶活性显著降低(P<0.05、0.01)。结论 早期糖尿病小鼠睾丸中睾酮合成关键酶的表达显著降低。
[Key word]
[Abstract]
Objective Type 1 diabetes mice model was established to investigate the changes of key enzymes involved in testosterone synthesis in testes of early diabetic mice. Methods Tatolly 20 male C57 mice were randomly divided into two groups:control and diabetic groups, and the diabetes mice were ip administered with a single dose of 150 mg/kg Streptozotocin. Four weeks after confirmation of diabetic model, the serum and testis were collected for further study. The qRT-PCR method was used to measure the expression of LHR and steroidogenesis synthetase StAR, P450scc, 3β-HSD6, P450c17a1, and 17β-HSD3 mRNA. ELISA assay was performed to measure the levels of testosterone and luteinizing hormone (LH) in serum, and the enzymatic activities of 3β-HSD1, 1P450c17 and 17β-HSD3 in testis tissue. Results Compared to control group, the levels of testosterone and LH of diabetic group declined significantly (P<0.05) after four weeks. The mRNA levels of LHR, StAR, CYP11a1, Hsd3b6, CYP17a1 and Hsd17b3, and enzymatic activities of 3β-HSD6, P450c17 and 17β-HSD3 were also decreased significantly compared with control group (P<0.05, 0.01 and 0.001). Conclusion Expression of key enzymes of testosterone synthesis in testis of early diabetic mice decreases significantly.
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[基金项目]
黑龙江省自然科学基金项目(LC201440);黑龙江省普通本科高等学校青年创新人才培养计划项目(UNPYSCT-2015110);黑龙江省大学生创新创业训练计划项目(201410229019);牡丹江市科学技术计划项目(Z2015s0038)