[关键词]
[摘要]
目的 建立毛郁金药材中不同成分同时测定的一测多评法。方法 以毛郁金中莪术二酮为指标,建立莪术二醇、ocathydro-1,4-dihydroxy-1,4-dimethyl-7-(propan-2-ylidene)azulen-5(1H)-one、原莪术醇和姜黄素的相对校正因子,在考察相对校正因子的重现性后,进一步采用外标法对一测多评法所计算出的成分含量进行验证。结果 10批毛郁金药材中,各成分采用相对因子计算的含量与采用外标法计算的结果无显著差异。结论 以莪术二酮为参照物,采用一测多评测定和计算其他4种成分含量的方法是可行的,结果较为准确。
[Key word]
[Abstract]
Objective To develop a method of quantitative analysis of multi-components by single marker (QAMS) for simultaneously determining five compounds in Curcumae Aromaticae Radix. Methods An HPLC method was developed as QAMS to determine curcuma diol, ocathydro-1,4-dihydroxy-1,4-dimethyl-7-(propan-2-ylidene)azulen-5(1H)-one, original curcumol and curcumin in Curcumae Aromaticae Radix, using curdione as intermal reference substance, and the relative correction factor (RCF) of the four components was determined by HPLC with good reproducibility. Their contents in 10 batches of samples, collected from different areas, were determined by both external standard method and QAMS. Result No significant differences were found in the quantitative results of four compounds in 10 batches of Curcumae Aromaticae Radix determined by external standard method and QAMS. Conclusion It is feasible and suitable to evaluate the quality of Curcumae Aromaticae Radix by QAMS.
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[基金项目]
国家新药创制项目(2014ZX09101-021-001)