目的 探讨白藜芦醇（Rsv）对咪喹莫特（IMQ）诱导的银屑病样皮肤损伤的改善作用及其作用机制。方法 BALB/c小鼠随机分为4组：对照组、Rsv组、模型（IMQ）组、Rsv治疗（IMQ+Rsv）组，每组6只；涂抹IMQ诱导BALB/c鼠耳部银屑病样皮肤损伤模型，连续8 d，同时涂抹20 mg Rsv予以治疗；观察红斑及银屑的多少、游标卡尺测量耳厚度；HE染色观察组织改变；实时荧光定量（qRT-PCR）检测组织角蛋白17（K17）、IL-17A、IL-19及IL-23 mRNA表达；Western blotting检测K17蛋白表达。结果 与模型组比较，Rsv治疗组小鼠红斑面积明显减少，表皮鳞屑减少，耳缘厚度显著降低；HE染色结果表明，Rsv治疗组耳缘组织表皮厚度明显减轻，炎细胞浸润减少；与对照组比较，模型组K17、IL-17A、IL-19及IL-23 mRNA水平、K17蛋白表达明显升高，Rsv治疗后K17、IL-17A、IL-19 mRNA水平、K17蛋白表达显著降低。结论 白藜芦醇通过抑制K17及炎症分子表达，改善IMQ诱导的银屑病样皮肤损伤。

Objective To observe the therapeutic effect of resveratrol (Rsv) on psoriasis like skin damage induced by imiquimod (IMQ) and study its mechanism. Methods BALB/c mice were randomly divided into four groups:control group, Rsv group, model (IMQ) group and Rsv treatment (IMQ+Rsv) group with six mice in each group. Psoriasis like skin model induced by smearing IMQ in the mice's ear for 8 d, 20 mg Rsv cream were gave at the same time for treatment. Observe erythema and psoriasis degree, and measure ear thickness with vernier caliper. HE staining was used to observe tissue change, real time quantitative PCR (qRT-PCR) was used to detect K17, IL-17A, IL-19 and IL-23 mRNA and Western blotting were used to detected K17 protein expression in tissues. Results Compared with the model group, mice erythema area and the thickness of epidermal scale decreased significantly in Rsv treatment group; HE staining results showed that, Rsv significantly reduced ear marginal tissue epidermal thickness, and diminished severity of the psoriasis-like skin inflammation. qRT-PCR and Western blotting confirmed a Rsv dependent decrease of in K17, IL-17A and IL-19 in mRNA level and protein levels of K17. Conclusion Rsv improves psoriasis like skin damage by down-regulating expression of Keratin17.

陕西省自然科学基础研究面上项目（2016JM8046，2014JQ2-8055，2016JM8047）；陕西省卫计委重点项目（2014A）；西安医学院校级重点建设学科资助项目（2015097）