[关键词]
[摘要]
目的 探讨润燥清肺膏的止咳、抗炎、抑菌的药效学作用。方法 将50 只小鼠及豚鼠各随机分为5 组,分别为模型组、阳性对照药急支糖浆组和润燥清肺膏高、中、低剂量组,采用氨水喷雾致咳小鼠模型及枸橼酸喷雾致咳豚鼠模型评价止咳作用;将60 只大鼠随机分为6 组,分别为正常组、模型组、阳性对照药急支糖浆组和润燥清肺膏高、中、低剂量组,采用脂多糖致急性支气管炎模型大鼠评价抗炎作用;将50 只小鼠随机分为5 组,分别为模型组、阳性对照药急支糖浆组和润燥清肺膏高、中、低剂量组,采用醋酸致小鼠腹腔毛细血管通透性增高及巴豆油致小鼠耳肿胀等模型评价其抗炎作用;采用体外抑菌实验,评价其抑菌作用。结果 润燥清肺膏高、中、低各剂量组豚鼠咳嗽潜伏期明显延长(P< 0.05、0.01)、咳嗽次数明显减少(P< 0.01);润燥清肺膏能减轻急性支气管炎大鼠模型黏膜上皮细胞的坏死和脱落,抑制黏膜层和黏膜下层炎性细胞浸润;润燥清肺膏高、中剂量组明显抑制醋酸所致小鼠腹腔毛细血管通透性的增高(P< 0.05、0.01);润燥清肺膏高、中、低各剂量组明显地抑制小鼠耳肿胀(P< 0.05、0.01)。润燥清肺膏对金黄色葡萄球菌、表皮葡萄球菌、肺炎克雷伯菌和大肠杆菌有不同程度的抑制作用。结论 润燥清肺膏对急性支气管炎模型大鼠支气管病变有抑制作用,具有明显的止咳、抗炎、抑菌作用。
[Key word]
[Abstract]
Objective To observe the main effects of the Ruzaoqingfei Gao in the treatment on asthma, cough and anti-inflammator. Methods Fifty rats were divided into 5 groups, the normal group, Ruzaoqingfei Gao high, middle, low, jizhi yrup, by with ammonia water spray determination of cough incubation period and Citrate spray induced cough model of guinea pigs; 60 big mice divided into 6 group, the normal group Ruzaoqingfei Gao high, middle, low dose group, jizhi syrup group or ammonium chloride, with the rat model of chronic bronchitis was induced with intratracheal instillation of lipopolysaccharide to observe the lung pathematology; 50 mice were divided into 5 groups, the normal group, Ruzaoqingfei Gao high, middle, low dose group, jizhi syrup group, acute inflammation model caused by dimathyl benzene and albumen.To evaluat bacteriostasis in vitro bacteriostasis experiment. Results Ruzaoqingfei Gao with high, medium and low dose groups of guinea pig cough obviously prolong the incubation period (P < 0.05, P < 0.01), less cough obviously (P < 0.01); The histopathological examination revealed Ruzaoqingfei Gao could reduce the chronic bronchitis rats with varying degrees of bronchial mucosa and submucosa infiltration of inflammatory cells, and reduce airway secretions; Ruzaoqingfei Gao with high, middle dose group significantly inhibited the increase of blood capillary permeability of the abdominal in mice caused by acetic acid (P < 0.05, P < 0.01); Ruzaoqingfei Gao with high, medium and low dose group obviously inhibit ear swelling in mice (P < 0.05, P < 0.01). With Ruzaoqingfei Gao on staphylococcus aureus and epidermis staphylococcus aureus, klebsiella pneumonia and escherichia coli, shows that there are different degrees of inhibition. Conclusion we confirmed the effects such as antitussive,anti-asthma and anti-inflammatory on animals of Ruzaoqingfei Gao.
[中图分类号]
[基金项目]
山东省科技发展计划项目(2014GSF119039)