目的 采用HPLC法测定红参提取物中α-亚麻酸、亚油酸的量,为红参的质量控制提供参考依据.方法 采用DIKMA Diamonsil C₁₈(250 mm×4.6 mm,5 μm)色谱柱,以乙腈-0.1%磷酸水(90:10)为流动相,体积流量1.0 mL/min,检测波长203 nm,柱温30 ℃.结果 在此条件下,α-亚麻酸、亚油酸与其他组分能得到良好的分离,α-亚麻酸、亚油酸的进样质量分别在0.066～0.33 μg(r＝0.999 9)、1.39～6.96 μg(r＝0.999 9)与各自峰面积积分值呈良好线性关系,专属性、精密度、稳定性、加样回收率均符合中药含量测定要求.结论 本方法可用于测定红参中的α-亚麻酸、亚油酸,为红参的质量监控提供简便、快速的方法.

Objective To determine the contents of α-linolenic acid and linoleic acid in red ginseng extract by HPLC, and to provide reference for the quality control of red ginseng. Methods Using DIKMA Diamonsil C₁₈ (250 mm × 4.6 mm, 5 μm) column, acetonitrile 0.1% phosphoric acid water (90:10) as mobile phase, flow rate of 1.0 mL/min, the detection wavelength was 203 nm. The column temperature was 30 ℃. Results Under this condition, α-linolenic acid, linoleic acid and other components could get good separation, and the sample injection contents of α-linolenic acid and linoleic acid respectively were in the range of 0.066—0.33 μg (r = 0.999 9), 1.39—6.96 μg (r = 0.999 9) and their peak area values showed a good linear relationship. The specificity, precision, stability, and recovery rate all met the requirements of content determination of Chinese materia medica. Conclusion This method can be used for the determination of α-linolenic acid and linoleic acid contents in red ginseng, providing the method is simple and fast for the quality control of red ginseng.

科技重大专项"重大新药创制"(2013ZX09402202)