[关键词]
[摘要]
目的 比较降压避风片新老配方的降压效果及作用特点.方法 选用原发性高血压大鼠(SHR)及其对照性WKY大鼠进行对比试验.实验动物分为8组:WKY大鼠组、SHR对照组、降压避风片老配方组高、中、低剂量(1.00、0.50、0.25 g药粉/kg)组、降压避风片新配方高、中、低剂量(1.00、0.50、0.25 g药粉/kg)组.降压试验采用大鼠无创血压测定仪对大鼠尾动脉进行血压测定,分为急性降压作用试验和慢性降压验.急性降压测定给药前、给药后1、3 h血压值;慢性降压测定连续给药后6、11、14 d血压值.连续给药14 d后,进行大鼠心脏超声心动试验,监测大鼠室间隔厚度、左室内径、左室后壁、射血分数、左室短轴缩短率、左心室心肌质量、左心室容量等;后腹主动脉取血,分别测定血清NO、总蛋白、白蛋白、球蛋白、丙氨酸氨基转移酶、天门冬氨酸氨基转移酶、尿素、肌酐、葡萄糖、三酰甘油、低密度脂蛋白和血浆内皮素、肾素活性及血管紧张素Ⅱ含量.结果 急性降压试验:各剂量组均可显著降低药后3 h的收缩压(P <0.01),新老配方相应剂量间无显著差异;慢性降压试验:SHR组心率显著快于WKY组,用药后,各剂量组均无显著降低心率的效果.结论 新老配方均可平稳降低SHR血压,降压作用确切.
[Key word]
[Abstract]
Objective To compare the antihypertensive effect and function characteristic of the old and new Jiangya Bifeng formula. Methods spontaneously hypertensive rats (SHR) and its controlled WKY rats were employed in the comparative study. Experimental animals were divided into 8 groups: WKY rats group, SHR control group, old Jiangya Bifeng formula of high, medium and low dose group, new Jiangya Bifeng formula of high, medium and low dose group. Step-down test adopted the rat noninvasive blood pressure meter on rat tail artery blood pressure measurement, divided into acute antihypertensive effect test and chronic step-down test. In the acute antihypertensive study, blood pressure value were tested before the drug delivery and after 1, 3 h; In the chronic antihypertensive study, the blood pressure values were determined continuous dosing after 6, 11, 14 d. After 14 days continuous dosing,the level of Echocardiographic heart、IVS、LVID、LVPW、EF、FS、LV Mass、LV Vol were determined.After posterior abdominal aortic blood, blood were tested: Serum NO, total protein, albumin, globulin, alanine aminotransferase, aspartate aminotransferase, urea, creatinine, glucose, triglycerides, low-density lipoprotein and plasma endothelin, renin activity and angiotensin Ⅱ content. Results acute anti-hypertension test: 3 h after Jiangya Bifeng formula administration the blood pressure were significantly reduced. However, there was no difference between the old and new formula (P <0.01); Chronic anti-hypertension test: The heart rate of SHR was significantly faster than WKY rats, after drugs administration, the heart rate were not significantly affected by both old and new Jiangya Bifeng formula. Conclusion the new and old Jiangya Bifeng formula could smoothly reduce the blood pressure, and both have a reliable antihypertensive effect.
[中图分类号]
[基金项目]