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[摘要]
目的 建立救心滴丸中华蟾酥毒基、脂蟾毒配基的定量方法。方法 采用高效液相色谱法,以C18色谱柱(250 mm×4.6 mm,5 μm);0.5%磷酸二氢钾溶液-乙腈(50:50)(用磷酸调pH值为3.2)为流动相;体积流量为1.0 mL/min,检测波长为296 nm。结果 华蟾酥毒基在0.092~1.472 μg呈良好的线性关系,r=0.999 7。平均回收率为101.4%,RSD值为1.0%。脂蟾毒配基在0.102~1.632 μg呈良好的线性关系,r=0.999 8。平均回收率为98.4%,RSD值为0.31%。结论 该方法简单、灵敏度高,可用于救心滴丸中华蟾酥毒基、脂蟾毒配基的定量测定。
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[Abstract]
Objective To develop an HPLC method for the determination of cinobufagin and resibufogenin in Jiuxin Dropping Pills. Methods The C18 colum (250 mm×4.6 mm, 5μm) was used with 0.5% potassium dihydrogen phosphate-acetonitrile (pH=3.2) (50:50) as a mobile phase. The flow rate was 1.0 mL/min. The detecting wavelength was 296 nm. Results Cinobufagin: the linear range of ferulic acid was 0.092—1.472 μg, r=0.9997, the average recovery rate was 101.4%. Resibufogenin: The linear range of ferulic acid was 0.102—1.632 μg, r=0.9998. The average recovery rate was 98.4%. Conclusion The method is simple, rapid, and accurate for the determination of cinobufagin and resibufogenin in Jiuxin Dropping Pills.
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