目的 建立测定宁嗽化痰丸中橙皮苷的高效液相色谱（HPLC）法。方法 采用美国的LabAlliance仪器，色谱柱为ZORBAX Eclipse XDB-C18（150 mm × 4.6 mm，5 μm）；流动相为甲醇-0.5%醋酸（39：61）；检测波长为284 nm，柱温40 ℃；体积流量0.8 mL/min。理论板数按橙皮苷计算应不低于3 000。结果 橙皮苷线性范围为0.020 9～0.209 0 mg/mL，平均加样回收率为99.86%，RSD值为1.88%。结论 该方法简单、快速、准确，可作为宁嗽化痰丸质量控制方法。

Objective To extablish an HPLC method to determine the content of hesperidin in Ningsou Huatan Pill. Methods The content of hesperidin in NingSou Huatan Pill was determined by HPCL method. The LabAlliance Apparatus from US was used, octadecyl silica bonded silica gel was used as packing agent; The mobile phase was methanol-0.5% acetic acid (39:61), with the wavelength of 284 nm, column temperature of 40 ℃, and flow rate of 0.8 mL/min. The number of theoretical plates was no lower than 3 000 in terms of hesperidin. Results The linear scope of hesperidin was 0.0209—0.2090 mg/mL, sample recovery rate in average was 99.86%, and the RSD value was 1.88%. Conclusion The method with HPLC is simple, rapid, and accurate, thus can be used as quality control method of Ningsou Huatan Pill.