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[摘要]
目的 考察五倍子不同产地(河北曲阳、浙江嘉兴、湖南桑植、陕西西乡、云南凤县)的体外抗菌作用。方法 五倍子醇提浸膏用倍半稀释法稀释成不同浓度药液,采用管碟法测定各产地五倍子的最小抑菌浓度(MIC)。结果 河北、浙江、湖南、陕西、云南所产五倍子醇提浸膏对金黄色葡萄球菌的MIC依次为6.25、1.562 5、1.562 5、3.125、0.781 25 mg/L;对大肠杆菌的MIC依次为3.125、12.5、6.25、6.25、3.125 mg/L;对白色念珠菌的MIC依次为6.25、1.562 5、3.125、1.562 5、3.125 mg/L;对绿脓杆菌的MIC依次为12.5、6.25、6.25、6.25、3.125 mg/L。结论 河北、浙江、陕西、云南、湖南所产五倍子均有抑菌作用,其中云南产五倍子醇提物对金黄色葡萄球菌、大肠杆菌、绿脓杆菌抑菌作用最强,陕西、浙江产五倍子醇提物对白色念球菌的抑菌作用强。
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[Abstract]
Objective To evaluate the in vitro antibacterial activity of ethanol extract from Galla Chinensis (EEGC) from different origins (Hebei, Zhejiang, Hunan, Shaanxi, and Yunnan provinces). Methods EEGC was diluted into different concentration by half times dilution method. Minimum inhibitory concentration (MIC) of Galla Chinensis from different origins was determined by cylinder-plate method. Results The MIC values of EEGC from above five provinces against Staphylococcus aureus were 6.25, 1.562 5, 1.562 5, 3.125, and 0.781 25 mg/L. The MIC values against Escherichia coli were 3.125, 12.5, 6.25, 6.25, and 3.125 mg/L. The MIC values against C. albicans were 6.25, 1.562 5, 3.125, 1.562 5, and 3.125 mg/L. The MIC values against Pseudomonas aeruginosa were 12.5, 6.25, 6.25, 6.25, and 3.125 mg/L. Conclusion Galla Chinensis from the five provinces all have bacteriostatic action, EEGC from Yunnan province has the strongest bacteriostatic action against S. aureus, E. coli, and P. aeruginosa. In addition, EEGC from Shaanxi and Zhejiang province exhibites the notable bacteriostatic action against C. albicans.
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