目的 探讨不同剂型的垂盆草对大鼠四氯化碳（CCl₄）急性肝损伤模型的保护作用。方法 Wistar大鼠80只，随机分成正常组、模型组、鲜垂盆草榨汁高、低剂量组及鲜垂盆草煎煮组、干垂盆草煎煮组、垂盆草颗粒组、垂盆草配方颗粒组。ig给药，1 次/d，连续7 d。第7天，除正常组外，给予大鼠ip 50% CCl₄橄榄油造模。观察大鼠的一般情况，检测肝功能指标总胆红素（Tbil）、丙氨酸氨基转移酶（ALT）、门冬氨酸氨基转移酶（AST）及肝组织丙二醛（MDA）、超氧化物歧化酶（SOD）、一氧化氮（NO）的含量和肝脏组织学变化情况。结果 干垂盆草煎煮组可显著降低大鼠肝质量。干垂盆草煎煮组、垂盆草颗粒组、垂盆草配方颗粒组均能降低大鼠血清ALT水平，干垂盆草煎煮组能降低大鼠血清AST水平。干垂盆草煎煮组、垂盆草颗粒组和垂盆草配方颗粒组均可降低大鼠肝组织MDA、NO的含量，提高大鼠肝组织SOD活力。干垂盆草煎煮组、垂盆草颗粒组、垂盆草配方颗粒组显著改善肝小叶细胞的变性和炎症坏死，减轻汇管区的炎细胞浸润。结论 干垂盆草煎煮液、垂盆草颗粒、垂盆草配方颗粒可保护CCl₄导致的大鼠急性肝损伤，且起到抗脂质过氧化的作用。

Objective To explore the preventive effect of different preparations of Sudum sarmentosum on the model of carbon tetrachloride (CCl₄)-induced acute liver injury in rats. Methods Purebred 80 Wistar rats were randomly divided into normal, model, the high- and low-dose fresh sarmentosum juice groups, the decoction of fresh sarmentosum, the decoction of sarmentosum, the sarmentosum granules, and the sarmentosum formula granules groups. Gavage started from the first day of the experiment, once a day, for consecutive 7 d. All rats in each group accepted ip injection of 50% CCl₄ olive oil except the normal group on the day 7. General changes of vital signs, the liver function (Tbil, ALT, and AST), levels of MDA, SOD, and NO in liver tissue, and liver histology examination were observed. Results The dry sarmentosum decoction group could significantly reduce the weight of liver. The dry sarmentosum decoction group, the sarmentosum granules group and the sarmentosum formula granules group could reduce the levels of ALT in serum of rats. The dry sarmentosum decoction group could also reduce the levels of AST in serum of rats. The dry sarmentosum decoction group, the sarmentosum granules group, and the sarmentosum formula granules group could reduce the levels of MDA and NO and improve the levels of SOD in liver tissue of rats. The dry sarmentosum decoction group, the sarmentosum granules group and the sarmentosum formula granules group could significantly improve the hepatic lobule cell degeneration inflammation and necrosis, reduce the infiltration of inflammatory cell in portal areas. Conclusion The dry sarmentosum decoction solution, the sarmentosum granules, and the sarmentosum formula granules have a protective effect on rats with CCl₄-induced acute liver injury and show the anti-lipid peroxidation.

国家中医药管理局重点学科建设—中医传染病学