目的 通过给予大鼠伸筋草提取物，探讨其中主要活性成分玉柏碱的药动学特征。方法 利用RP-HPLC法，以Centurysil C₁₈ EPS为色谱柱，甲醇-水（61∶39）为流动相；紫外检测器测定大鼠ig伸筋草提取物后血浆中玉柏碱的血药浓度，计算药动学参数。结果 玉柏碱在大鼠体内呈二室模型分布，线性范围为0.354～14.16 μg/mL，r＝0.999 8。日内和日间精密度均小于5%。玉柏碱口服给药后在大鼠体内的主要药动学参数为：t_1/2为2.681 h，t_max为0.75 h，C_max为6.309 mg/L，AUC_(0→t) 为16.626 mg/L，AUC_(0→∞) 为18.798 mg/L。结论 本方法适用于大鼠血浆中玉柏碱的检测及其体内药动学研究。

Objective To study the in vivo pharmacokinetic characteristics of α-obscurine in rats after the ig administration of Lycopodium japonicum extract (LJE). Methods The RP-HPLC was used to monitor the plasma concentration of α-obscurine in rats after the ig administration of LJE. Methanol-water (61:39) as the mobile phase on Centurysil C₁₈ EPS column. The pharmacokinetic parameters were estimated by the DAS2.0 computer program. Results The concentration-time profiles were fitted with an open two compartment model system. The main pharmacokinetic parameters were t_1/2 2.681 h, t_max 0.75 h, C_max 6.309 mg/L, AUC_(0→t) 16.626 mg/L, AUC_{( 0→∞)} 18.798 mg/L. Conclusion The HPLC method is appropriate for the determination and pharmacokinetic study on α-obscurine in rat plasma after the ig administration of LJE.

国家863课题（2004 AA.2Z3730-13）