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[摘要]
目的 探讨不同品系栝楼果皮抗心肌梗死的药效差异,为栝楼优良品种选育提供参考。方法 选择道地产区3个主要品系的栝楼果皮作为研究材料。60只大鼠随机分成对照组、丹参片(阳性药对照)组以及1、2、3号栝蒌皮组。结扎大鼠冠状动脉,制备冠心病急性心肌梗死模型;对照组实行假手术。栝蒌皮制成水煎剂ig给药,丹参片为阳性对照药。氯化三苯四氮唑染色,测定心肌梗死率;生理记录仪记录心电图变化,检测心脏功能;生化试剂盒检测血浆中肌酸激酶(CK)、肌酸激酶同工酶(CKMB)、谷草转氨酶(AST)、α-羟基丁酸脱氢酶(LDH)和乳酸脱氢酶(AST)活性,确定心肌细胞坏死程度;测定心肌组织丙二醛(MDA)含量及超氧化物歧化酶(SOD)活性,明确大鼠体内清除氧自由基的能力。结果 与模型组相比,3个栝蒌皮组心电图△ST值、心肌梗死面积、血浆5种心肌酶活性、心肌组织MDA含量均显著降低(P<0.05或0.01),与丹参片组比较差异无显著性;而心肌组织中SOD活性明显升高(P<0.05);其中上述9种指标以2号栝蒌组与模型组差异最为显著。结论 3个品系栝蒌皮均有显著降低急性心肌缺血模型大鼠心肌梗死率、维护心脏功能、防止心肌细胞坏死、增强其清除氧自由基的能力。其中2号品系栝蒌皮效果最显著。不同品系栝蒌皮干预急性心肌梗死效果有差异,2号品系栝蒌皮保护效果优于其他品系。
[Key word]
[Abstract]
Objective To investigate the pharmacodynamic differences of anti-miocardial infaration among different species of Trichosanthes pericarpium, which could provide the reference for breeding the fine varieties of T. pericarpium. Methods Pericarps of three species of T. pericarpium from geo-authentic areas were selected as objects. Sixty Wistar rats were randomly divided into the control, model, Danshen Pian group (positive control group), trichosanthes 1, trichosanthes 2, and trichosanthes 3 groups. Acute miocardial ischemia model was made through the ligation of coronary artery. The control group was Sham operated. The pericarp of T. pericarpium was prepared as decoction and ig administered and Danshen Pian is the positive control group. To measure the infarction area, myocardial tissue was stained with triphenylte trazoliam chloride (TTC). The degree of myocardial infarction was evaluated by electrocardiograph (ECG). To evaluate the myocardial injury, the serum levels of creatine-kinase (CK), creatine-kinase-MB (CKMB), lactate dehydrogenase (LDH), and aspartate-amino transferase (AST) were detected. Moreover, the in vivo activity of scavenging oxygen free radical was detected through detecting the content of malondialdehyde (MDA) and superoxide dismutase (SOD) in myocardial tissue. Results Compared with those in the model group, the △ST value, the myocardial infarction rate, and the serum activity of CK, CK-MB, LDH, AST, and α-HBDH of rats in three trichosanthes groups were obviously lowered (P < 0.05 or P < 0.01). There was no significant difference compared with Danshen Pian group. The activity of SOD in the myocardia increased observably (P < 0.05). Among these nine indexes, the most observable changes could be detected in the trichosanthe 2 group of rats. Conclusion All the three species of T. pericarpium could significantly reduce the myocardial infarction rate of acute myocardial ischemia model rats, maintain the function of heart, prevent myocardial necrosis, and increase the scavenging activity of free radical. The protective effect of the three varieties of T. pericarpium on the acute myocardial ischemia of rats is significant, but the effect of the trichosanthe 2 variety is the most obvious.
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[基金项目]
济南市科技发展计划项目资助(200906028);上海市科学技术委员会项目资助(11HX1171400)