目的 探讨注射用黄芪多糖药物血清在体外对耐顺铂（DDP）人肺腺癌A549/DDP细胞株的耐药逆转作用。方法 通过建立小鼠肿瘤模型，运用血清药理学方法，以人肺腺癌敏感细胞A549和耐药细胞A549/DDP为研究对象，采用细胞毒实验（MTT）方法，探讨注射用黄芪多糖对耐顺铂人肺腺癌细胞A549/DDP的耐药逆转作用。结果 注射用黄芪多糖药物血清高、中、低剂量组分别与DDP合用时对A549/DDP细胞的耐药逆转倍数分别为1.24、1.41、1.34；且中、高剂量组与阳性对照DDP组比较差异有统计学意义（P＜0.05）。结论 注射用黄芪多糖能明显增加A549/DDP耐药细胞对DDP的敏感性，提高细胞死亡率，具有耐药逆转作用。

Objective To investigate the resistance reversal effect of the drug serum of astragalus polysaccharides for injection (API) in vitro on cisplatin-resistant human lung adenocarcinoma cell line A549/DDP. Methods The model of tumor-bearing mice was established, and using serum pharmacology method and taking human lung adenocarcinoma sensitive cell A549 and drug-resistant cell A549/DDP as the research objects, the resistance reversal effect of API on cisplatin-resistant human adenocarcinoma cells A549/DDP was studied in cell toxicity experiment by MTT method. Results The resistance reversal multiples of high-, mid-, and low-dose groups of API drug serum to A549/DDP cells were 1.24, 1.41, and 1.34, respectively; The data in mid- and high-dose groups had significant difference (P < 0.05) compared with DDP (positive control) group. Conclusion API could obviously increase the sensitivity of A549/DDP cells on DDP and improve the cell mortality. API has the resistance reversal effect.

天津市应用基础及前沿技术研究计划（青年基金项目）（12JCQNJC07500）；天津市高等学校科技发展基金计划项目（20110223）