[关键词]
[摘要]
目的 建立通光藤药材及消癌平片中通光藤苷A的HPLC测定方法。方法 色谱柱为Sinochrom ODS-BP分析柱(250 mm×4.6 mm,5 μm),流动相为乙腈-0.1%磷酸(41∶59);检测波长230 nm。结果 通光藤苷A在20.2~404.0 μg与峰面积积分值呈良好的线性关系,r=0.999 95,平均加样回收率为96.6%,RSD值为2.19%(n=6)。结论 该方法简单,结果准确,稳定性好,重现性高,可用于通光藤药材及消癌平片定量测定及质量控制。
[Key word]
[Abstract]
Objective To develop an HPLC method for determination of tenacissosides A in Marsdenia tenacissima and Xiaoaiping Tablets. Methods Sinochrom ODS-BP column was used (250 mm × 4.6 mm, 5 μm), the mobile phase consisted of acetonitrile-0.1% (41:59), and the UV detection wavelength was at 230 nm. Results Good linear correlation for tenacissosides A was found in the concentration range of 20.2―404.0 μg (r = 0.999 95). The average recovery rate was 96.6%, with RSD of 2.19% (n = 6). Conclusion The method is simple, accurate, and stable which is fit for determination and quality control of Marsdeniae Tenacissimae Caulis and Xiaoaiping Tablets.
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[基金项目]
通关藤种质鉴定与药材质量相关性研究(2011011035-1)