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[摘要]
目的 建立HPLC测定清胃黄连丸中小檗碱的方法。方法 色谱柱Phenomenex Luna C18(250 mm×4.6 mm,5 μm);流动相为乙腈-0.03 mol/L磷酸二氢钾(35∶65);检测波长345 nm;体积流量1.0 mL/min。结果 线性范围为25.5~510.0 ng(r=0.999 9),平均回收率为99.39%,RSD为0.87%。结论 该方法稳定、可靠,可作为清胃黄连丸的质量控制方法。
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[Abstract]
Objective To establish an HPLC method to determine berberine in Qingwei Huanglian Pills. Methods Berberine was assayed on a Phenomenex Luna C18 column (250 mm × 4.6 mm, 5 μm); The mobile phase composed of acetonitrile-0.03 mol/L potassium dihydrogen phosphate (35:65) at a flow rate of 1.0 mL/min; The determination wavelength was 345 nm. Results The calibration curves were linear in the range of 0.025 5—0.510 0 μg (r = 0.999 9) for berberine. The average recoveries (n = 6) of berberine was 99.39% (RSD = 0.87%). Conclusion The method is proved to be simple and reliable and could be used for quality control of Qingwei Huanglian Pills.
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