[关键词]
[摘要]
目的:建立血府逐瘀胶囊原料药材红花中羟基红花黄色素A和芦丁,川芎中生物碱类成分川芎嗪和有机酸类物质阿魏酸的定量的测定方法,从而建立多成分的定量标准。方法:采用HPLC法,色谱条件(红花):C18色谱柱,以甲醇-0.4%磷酸水溶液为流动相进行梯度洗脱,体积流量 0.5 mL/min,柱温 40 ℃。色谱条件(川芎):C18色谱柱,以甲醇-1%冰醋酸水溶液(32︰68)为流动相,体积流量 1.0 mL/min,柱温30 ℃。结果: 平均回收率分别为羟基红花黄色素A 97.2%、RSD为2.3%(n=6);芦丁99.6%、RSD为3.2%(n=6);川芎嗪100.9%、RSD为2.2%(n=6)阿魏酸98.6%、RSD为2.6%(n=6)。结论:本方法简便、快速、准确,可作为血府逐瘀胶囊的原料药材的质量控制方法。
[Key word]
[Abstract]
Objective: To establish the HPLC methods for the determination of hydroxysafflor yellow A and rutin in raw material and ligustrazine and ferulic acid in Ligusticum chuanxiong of Xuefuzhuyu Capsula and quantitative criteria for multi-component. Methods: The HPLC was applied with a C18 column for the determination of Carthamus tinctorius by gradient elution using methanol -0.4% phosphonic acid solution as the mobile phase. The flow rate was 0.5 mL/min and the temperature of column was 40?C. The HPLC was applied with a C18 column for the determination of L. chuanxiong using methanol -1.0% acetic acid solution (32︰68) as the mobile phase. The flow rate was 1.0 mL/min and the temperature of column was 30?C. Results: The average recovery rates were 97.2% ,99.6% ,100.9% , and 98.6%, RSD were 2.3%, 3.2%, 2.2%, and 2.6% for,hydroxysafflor yellow A and rutin, ligustrazine and ferulaic acid, respectively (n=6). Conclusion: The method is simple ,rapid, and accurate. It can be used for the quality control of Xuefuzhuyu Capsula.
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[基金项目]
天津市科技创新专项资金项目(06FZZDSH00404)