目的 采用不同模型评价海马补肾丸免疫调节作用和抗应激作用。方法 健康昆明种小鼠随机分为对照组、模型组、香菇菌多糖（100 mg/kg）组、龟龄集（0.24 g/kg）组和海马补肾丸0.55、1.10、2.20 g/kg组，每天ig给药1次，连续14 d。①采用注射盐酸环磷酰胺致小鼠免疫抑制模型，给药结束尾iv 25%印度墨汁，计算廓清指数（κ）和吞噬指数（α），观察小鼠肝指数、脾指数和胸腺指数；②采用注射盐酸环磷酰胺致小鼠免疫抑制模型，ip 5%鸡红细胞悬液0.3 mL致敏，观察各组血清溶血素生成量；③给药第8～10天，除对照组外，剩余各组开始ip环磷酰胺80 mg/kg，连续3 d，继续给药，至给药第14天眼眶静脉取血，测定白细胞数及免疫球蛋白IgG、IgM、IgA含量；④SD大鼠随机分为对照组、模型组、单硝酸异山梨酯片20 mg/kg、龟龄集0.12 g/kg和海马补肾丸低、中、高剂量（0.28、0.56、1.12 g/kg）组，每天ig给药1次，连续14 d，观察海马补肾丸对运动疲劳大鼠游泳时间及运动后对血清尿素氮（BUN）、乳酸（LD）、肝糖原水平的影响；⑤健康昆明种小鼠随机分为对照组、模型组、单硝酸异山梨酯片（40 mg/kg）组、龟龄集（0.24 g/kg）组和海马补肾丸低、中、高剂量（0.55、1.1、2.2 g/kg）组，每天ig给药1次，连续5 d，于末次给药后1 h，采用注射异丙肾上腺素致小鼠缺氧模型，将小鼠置于密封瓶中，观察存活时间。结果 与模型组比较，海马补肾丸2.2 g/kg显著增加α及κ（P<0.05、0.01），海马补肾丸1.1、2.2 g/kg剂量显著增加脾、胸腺系数（P<0.05、0.01）；海马补肾丸各剂量组显著增加血清溶血素生成量（P<0.05、0.01）；海马补肾丸2.2 g/kg剂量组显著增加白细胞数量及免疫球蛋白IgG、IgM、IgA的含量（P<0.05）；海马补肾丸0.56、1.12 g/kg剂量显著延长大鼠力竭游泳时间（P<0.05、0.01），1.12 g/kg剂量能显著地减少血清中BUN、LD含量，减少肝糖原消耗（P<0.05、0.01）；海马补肾丸1.1、2.2 g/kg剂量能显著延长小鼠缺氧存活时间（P<0.05、0.01）。结论 海马补肾丸具有明显免疫增强和抗应激作用。
Objective Different models were used to evaluate the immunomodulatory effect and anti-stress effect of Haima Bushen Pills. Methods The healthy Kunming mice were randomly divided into control group, model group, lentinan group (100 mg/kg), Guilingji group (0.24 g/kg) and Haima Bushen Pills group (0.55, 1.10 and 2.20 g/kg). The mice were ig given relevant drugs once a day for 14 days. ① Immunosuppressive model of mice was induced by cyclophosphamide hydrochloride injection. Indian ink of 25% was iv at the end of administration. Clearance index (κ) and phagocytic index (α) were calculated. Liver index, spleen index and thymus index of mice were observed. ② Immunosuppressive model of mice was induced by injection of cyclophosphamide hydrochloride, and sensitized by 0.3 mL of IP 5% chicken erythrocyte suspension. Serum hemolysin production was observed in each group. ③ On the 8th to 10th day of administration, except the control group, the remaining groups began ip cyclophosphamide 80 mg/kg for 3 consecutive days. The orbital vein blood was collected on the 14th day of administration, and the number of white blood cells and the contents of immunoglobulin IgG, IgM and IgA were measured. ④ SD rats were randomly divided into control group, model group, isosorbide mononitrate tablets 20 mg/kg, Guilingji 0.12 g/kg and low, medium and high dose groups (0.28, 0.56, 1.12 g/kg) of Hippocampus Kidney-tonifying Pills. The effects of Hippocampus Kidney-tonifying Pills on the levels of serum urea nitrogen (BUN), lactic acid (LD) and hepatic glycogen in exercise-fatigue rats were observed once a day for 14 days. ⑤ Healthy Kunming mice were randomly divided into control group, model group, isosorbide mononitrate tablet (40 mg/kg), Guilingji (0.24 g/kg) group and Hippocampus Kidney-tonifying Pills (0.55, 1.1, 2.2 g/kg) group. The mice were given Ig once a day for 5 days. One hour after the last administration, the mice were injected with isoprenaline to induce hypoxia. The mice were placed in sealed bottles for observation of survival time. Results Compared with model group, 2.2 g/kg of Haima Bushen Pills significantly increased α and κ (P<0.05 and 0.01), 1.1 and 2.2 g/kg of Haima Bushen Pills significantly increased spleen and thymus coefficient (P<0.05 and 0.01), and each dose of Haima Bushen Pills significantly increased serum hemolysin production (P<0.05 and 0.01); 2.2 g/kg of Haima Bushen Pills significantly increased the number of white blood cells and the IgG, IgM and IgA (P<0.05); the dosage of Haima Bushen Pills (0.56, 1.12 g/kg) significantly prolonged the exhaustive swimming time of rats (P<0.05, 0.01); the dosage of 1.12 g/kg could significantly reduce the contents of BUN and LD in serum and the consumption of hepatic glycogen (P<0.05, 0.01); the dosage of Haima Bushen Pills (1.1, 2.2 g/kg) could significantly prolong the hypoxic survival time of mice (P<0.05, 0.01). Conclusion Haima Bushen Pills has obvious immune enhancement and anti - stress effect.