目的 基于自然杀伤（NK）细胞相关文献分析探究NK细胞的研究现状、热点和发展趋势。方法 以中国学术期刊全文数据库（CNKI）、维普生物医学数据库（VIP）、万方数据库（Wanfang Data）、PubMed和Web of Science等中英文数据库收录的NK细胞相关的文献为研究对象，采用CiteSpace6.2.R2软件进行分析。结果 共纳入11 653篇与NK细胞相关的中英文文献，文献年发表量总体逐渐上升，主要作者有周忠海、刘军权、Wang Y等，主要研究机构有中国科学技术大学免疫学研究所、上海中医药大学和Tabriz University of Medical Sciences等，高频关键词主要有免疫功能、免疫治疗、肿瘤、免疫调节、肿瘤微环境等。结论 当前NK细胞研究热点主要集中于其抗肿瘤应用，利用NK细胞接合器杀伤肿瘤显示出更好的安全性与疗效，为NK细胞免疫疗法提供重要意义；在NK细胞培养方法方面的关注较少，日后可挖掘能够有效激活NK细胞的中药活性成分，增加联合中药培养NK细胞的方法及机制研究，为培养NK细胞提供新思路与新方向，同时促进中药理论与免疫细胞的融合发展。

Objective To explore the current research status, hotspots and development trends of natural killer cells (NK cells) based on the analysis of NK cells related literature. Methods Literature related to NK cells included in Chinese and English databases, such as CNKI, VIP Database, Wanfang Database, PubMed and Web of Science, were used as the study object and analyzed by CiteSpace 6.2.R2 software. Results A total of 11 653 NK cell-related papers in English and Chinese were included, with an overall gradual increase in the annual publication volume of the literature. The main authors include Zhou Zhonghai, Liu Junquan, Wang Y, and others, and the main research institutes were the Institute of Immunology, University of Science and Technology of China, the Shanghai University of Traditional Chinese Medicine, and the University of Medical Sciences in Tabriz, etc. The main high-frequency keywords are immune function, immunotherapy, tumor, immune regulation, tumor microenvironment and so on. Conclusion The current hotspots of NK cells research mainly focuses on its anti-tumor application, and the use of NK cells splicer to kill tumors shows better safety and efficacy, which provides an important significance for NK cells immunotherapy; less attention has been paid to NK cells culture methods, and in the future, we can explore the active ingredients of traditional Chinese medicine that can effectively activate NK cells, and increase the research on the methods and mechanisms of NK cells culture with traditional Chinese medicine, which will provide new ideas and new directions for the cultivation of NK cells, and promote the integration and development of traditional Chinese medicine theory and immune cells at the same time.

R979.1

教育部产学合作协同育人项目（22097040193735）