目的 建立同时测定白蔹中没食子酸、原儿茶酸、槲皮素、儿茶素、白蔹素、白藜芦醇、大黄素、大黄素甲醚、齐墩果酸、羽扇豆醇、豆甾醇、胡萝卜苷、β-谷甾醇含量的方法，结合化学计量学、加权TOPSIS模型，评价不同产地白蔹的质量。方法 采用HPLC法，应用Prism RP C₁₈色谱柱，乙腈-0.2%磷酸为流动相，梯度洗脱，采用280、254和210 nm三波长测定18批白蔹中上述13个成分含量，同时检测醇溶性浸出物（ ESE）、总灰分和酸不溶性灰分。结合化学计量学、加权TOPSIS法综合评价白蔹的整体质量。结果 13个成分在65 min内完全分离，在一定质量浓度范围内的线性关系良好，平均加标回收率为96.94%～100.10%，RSD均＜2.0%； 18批白蔹中13个成分含量分别为（ 0.509±0.110）、（ 0.672±0.086）、（ 1.498±0.255）、（ 3.597±0.601）、（ 0.246±0.080）、（ 0.248±0.051）、（ 0.141±0.029）、（ 0.183±0.044）、（ 0.113±0.040）、（0.387±0.085）、（0.052±0.025）、（0.069±0.014）、（0.343±0.076） mg·g-1，ESE、总灰分和酸不溶性灰分含量分别为（20.0±2.0）%、（ 9.0±2.4）%和（ 2.2±0.8）%，16个指标含量差异均较大。化学计量学分析结果显示18批白蔹聚为3类，以VIP值＞1为阈值，筛选出儿茶素、槲皮素、羽扇豆醇、原儿茶酸和β-谷甾醇为区分18个不同产地白蔹的差异性标志物。因子分析（ FA）和加权TOPSIS法均显示安徽、河南、湖北产地的白蔹质量较优。结论 基于HPLC法同时测定白蔹中13个成分含量方法，该方法准确可靠，化学计量学与加权TOPSIS法科学直观，可用于评价白蔹的整体质量。

Objective To establish a method for simultaneous determination of gallic acid, protocatechuic acid, quercetin, catechin, trichosanthin, resveratrol, emodin, physcion, oleanolic acid, lupeol, stigmasterol, daucosterol and β-sitosterol in Ampelopsis radix. and to evaluate the quality of A. radix from different origins by chemometrics and weighted TOPSIS model. Methods HPLC was used with a Prism RP C₁₈ column and a mobile phase of acetonitrile-0.2% phosphoric acid with gradient elution. The contents of the 13 components were determined at 280, 254 and 210 nm. The ethanol-soluble extractives (ESE), total ash and acid-insoluble ash were also determined. The overall quality of A. radix was comprehensively evaluated by chemometrics and weighted TOPSIS method. Results The 13 components were completely separated within 65 min. Good linear relationships were obtained within the tested concentration ranges. The average recovery rates were 96.94% to 100.10% with RSD all less than 2.0%. The contents of the 13 components in 18 batches of A. radix were (0.509 ±0.110), (0.672 ±0.086), (1.498 ±0.255), (3.597 ±0.601), (0.246 ±0.080), (0.248 ±0.051), (0.141 ±0.029), (0.183 ±0.044), (0.113 ±0.040), (0.387 ±0.085), (0.052 ±0.025), (0.069 ±0.014), (0.343 ±0.076) mg·g-1, respectively. The contents of ethanol-soluble extractives (ESE), total ash and acid-insoluble ash were (20.0 ±2.0)%, (9.0 ±2.4)% and (2.2 ±0.8)%, respectively. The contents of the 16 indicators varied greatly. Chemometrics analysis showed that the 18 batches of A. radix were clustered into 3 categories. With VIP value > 1 as the threshold, catechin, quercetin, lupeol, protocatechuic acid and β- sitosterol were selected as the differential markers for distinguishing A. radix from different origins. Both factor analysis and weighted TOPSIS method indicated that the quality of A. radix from Anhui, Henan and Hubei was relatively superior. Conclusion The HPLC method for simultaneous determination of 13 components in A. radix is accurate and reliable. Chemometrics and weighted TOPSIS method are scientific and intuitive, and can be used to evaluate the overall quality of A. radix.

R286.2

河南省医学教育研究项目（WJLX2024254）；河南省医学教育研究项目（WJLX2020326）；湖北省高等学校优秀中青年科技创新团队计划项目（T2023039）