目的 探讨苍术-白术药对及各单味药醇提物对葡聚糖硫酸钠（ DSS）溶液诱导溃疡性结肠炎（ UC）的小鼠肠黏膜损伤的影响。方法 将81只Balb/c雄性小鼠随机分为对照组，模型组（ 3.5% DSS），苍术醇提物（ ALEE）低、高剂量（555、1 110 mg·kg^-1）组，白术醇提物（ AMEE）低、高剂量（ 555、1 110 mg·kg^-1）组，苍术-白术药对醇提物（ AL-AMEE）低、高剂量（ 555、1 110 mg·kg^-1）组，柳氮磺吡啶（ SASP，阳性药，250 mg·kg^-1）组，每组9只。在7 d实验中，除对照组外，其余所有组的小鼠均自由饮用3.5% DSS溶液，以建立UC模型。造模第2天开始ig给药，每天1次，连续7 d。每天对小鼠的体质量和便血状况进行观察，计算疾病活动指数（ DAI）评分；测量小鼠结肠长度、计算脾脏系数；采用苏木精-伊红染色（ HE）法观察结肠组织的病理状态，并运用阿利新蓝/过碘酸雪夫染色（ AB/PAS）评估杯状细胞的数量；通过免疫组化法检测结肠组织中肿瘤坏死因子（ TNF）-α、白细胞介素（ IL）-1β、IL-6、基质金属蛋白酶（ MMP）-2和MMP-9蛋白的表达情况，实时荧光定量PCR（ qRT-PCR）法测定TNF-α、IL-1β、IL-6、结肠组织紧密连接蛋白1（ ZO-1）、闭合蛋白（ Occludin）mRNA的表达。结果 与模型组相比，各给药组体质量、结肠长度、DAI评分、杯状细胞数目显著增加（ P＜0.05、0.01），脾脏系数显著降低、结肠病理评分显著降低（ P＜0.05、0.01）；结肠组织中炎症因子TNF-α、IL-1β、IL-6转录水平及其蛋白表达，以及金属基质蛋白酶MMP-2、MMP-9蛋白表达水平显著降低（ P＜0.05、0.01），且紧密连接蛋白ZO-1、Occludin的mRNA表达水平显著升高（ P＜0.05、0.01），AL-AMEE组的作用最明显。结论 苍术-白术及其组成药味均能改善DSS诱导所引起的UC炎症，缓解结肠黏膜损伤，促进肠道屏障修复，且苍术-白术药对疗效更为显著。

Objective To investigate the effects of Atractylodes lancea-A. macrocephala pair and their individual components on intestinal mucosal injury in mice with ulcerative colitis (UC) induced by dextran sulfate sodium (DSS) solution. Methods Eightyone male Balb/c mice were randomly divided into a control group, a model group (3.5% DSS), low and high dose A. lancea ethanol extract (ALEE) groups (555, 1 110 mg·kg^-1), low and high dose A. macrocephala ethanol extract (AMEE) groups (555, 1 110 mg·kg^-1), low and high dose A. lancea-A. macrocephala ethanol extract (AL-AMEE) groups (555, 1110 mg·kg^-1), and a sulfasalazine (SASP, positive drug, 250 mg·kg^-1) group, with nine mice in each group. During the seven day experiment, except for the control group, all other groups of mice freely drank 3.5% DSS solution to establish the UC model. From the second day of modeling, intragastric administration was started once a day for 7 consecutive days. The body weight and bloody stool status of the mice were observed daily, and the disease activity index (DAI) score was calculated; The colon length and spleen coefficient of the mice were measured; The pathological state of the colon tissue was observed by hematoxylin-eosin staining (HE), and the number of goblet cells was evaluated by alcian blue/periodic acid-Schiff staining (AB/PAS); The expression of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, matrix metalloproteinase (MMP)-2 and MMP-9 proteins in the colon tissue was detected by immunohistochemistry, and the expression of TNF-α, IL-1β, IL-6, tight junction protein 1 (ZO-1), and Occludin mRNA in the colon tissue was determined by real-time fluorescence quantitative PCR (qRT-PCR). Results Compared with the model group, the body weight, colon length, DAI score, and the number of goblet cells in each treatment group were significantly increased (P < 0.05, 0.01), the spleen coefficient was significantly decreased, and the colon pathological score was significantly decreased (P < 0.05, 0.01); The transcriptional and protein expression levels of inflammatory factors TNF-α, IL-1β, IL-6, and the protein expression levels of MMP-2 and MMP-9 in the colon tissue were significantly decreased (P < 0.05, 0.01), and the mRNA expression levels of tight junction proteins ZO-1 and Occludin were significantly increased (P < 0.05, 0.01), with the AL-AMEE group showing the most significant effect. Conclusion A. macrocephala -A. lancea and their individual components can all improve the UC inflammation induced by SS, alleviate colonic mucosal injury, and promote intestinal barrier repair, and the effect of the A. macrocephala-A. lancea pair is more significant.

R285.5

湖北省教育厅科学研究计划重点项目（D20232001）