目的 分析胃癌患者中多聚腺苷二磷酸核糖聚合酶（PARP）族基因的表达情况及其与预后的关系，体外细胞实验研究胃复春胶囊醇提物（WFCEE）对胃癌SGC-7901细胞及PARP表达的影响。方法 基于TCGA/GTEx/GEO数据库分析PARP族基因表达及预后，蛋白互作网络分析PARP族基因的主要生物功能，并采用MCODE联合cytoHubba预测核心基因及其作用通路。将SGC-7901细胞与WFCEE（10、20、50 μg·mL^-1）共孵育，采用CCK-8细胞增殖与活性检测试剂盒检测细胞活力，流式细胞术Annexin V/PI双染色检测细胞凋亡，Western blotting法检测PARP及凋亡相关蛋白表达情况。结果 与正常组比较，胃癌患者PARP1、PARP4、PARP6、PARP9等基因表达有显著性差异；K-M曲线生存预后分析显示PARP平均表达量低组患者生存率高、中位生存时间更长。蛋白互作分析显示PARP家族主要与蛋白质ADP-核苷酸化、戊糖基团转移酶活性、糖基团转移酶活性、染色体上蛋白质定位、正或负向调节DNA代谢过程等过程相关，其中PARP1、PARP2、PARP3、PARP9、PARP10、PARP12、PARP14为核心基因，调节碱基切除修复、细胞凋亡、坏死性凋亡等信号通路。与对照组比较，WFCEE可时间、剂量相关性地抑制SGC-7901细胞活力并诱导凋亡（P＜0.05、0.01） ；20、50 μg·mL^-1组PARP蛋白表达量显著降低（P＜0.05）；各浓度组cleaved caspase-3、cleaved caspase-9、Bax蛋白表达量显著增加（P＜0.01、0.001），Bcl-2蛋白表达量显著降低（P＜0.01、0.001）。结论 PARP在胃癌组表达与正常组比较存在显著差异，并且与预后相关性显著，在胃癌临床应用方面有一定的价值；WFCEE可抑制胃癌细胞的增殖并诱导其凋亡，其作用机制可能与Bax/Bcl2-caspase3/9-PARP路径相关。

Objective To analyze the relationship between the expression of polyadenosine diphosphate-ribose polymerase (PARP) family genes and prognosis, and to verify the intervention of Weifuchun Capsules ethanol extract (WFCEE) on gastric cancer SGC- 7901 cells and PARP expression through cell in vitro experiments. Methods The expression and prognosis of PARP family genes were analyzed based on TCGA/GTEx/GEO database, protein interaction network analysis of the main biological functions of PARP family genes and using MCODE combined with cytoHubba to predict core genes and their pathways. Gastric cancer SGC-7901 cells were co-incubated with WFCEE (10, 20, and 50 μg·mL^-1) , cell viability was detected by Cell Counting Kit-8 (CCK-8) cell proliferation and activity detection kit, and cells were detected by flow cytometry Annexin V/PI double staining apoptosis, Western blotting method to detect the expression of PARP and related apoptosis proteins. Results There were significant differences in the expressions of PARP1, PARP4, PARP6, and PARP9 in gastric cancer group compared with control group. K-M curve survival prognosis analysis showed that patients with low average expression of PARP had a higher survival rate and a longer median survival time. Protein interaction analysis shows that the PARP family is mainly related to protein ADP-nucleotide, pentose group transferase activity, sugar group transferase activity, protein localization on chromosome, positive or negative regulation of DNA metabolism and other processes, among which PARP1/PARP2/PARP3/ PARP9/PARP10/PARP12/PARP14 are core genes, regulating base excision repair/apoptosis/ necroptosis and other signaling pathways. Experiments proved that, Compared with control group, WFCEE can inhibit the viability of SGC-7901 cells and induce apoptosis of SGC-7901 cells in a time- and dose-dependent manner (P < 0.05, 0.01, and 0.001). Western blotting shows that, WFCEE can down-regulate the expression of PARP and up-regulate the expressions of cleaved caspase-3, cleaved caspase-9, and Bax (P < 0.05, 0.01, and 0.001), and increase the expression of Bcl-2 (P < 0.01 and 0.001). Conclusion Compared with normal group, the expression of PARP in the gastric cancer group is significantly different and has a significant relationship with the prognosis, which has certain value in the clinical application of gastric cancer. Experiments have proved that WFCEE can inhibit the proliferation of gastric cancer cells and induce their apoptosis, and its mechanism of action is speculated to be the Bax/Bcl2-caspase3/9-PARP pathway.

浙江省科技计划项目（中药二次开发研究——以临床价值和质量提升为导向的“胃复春”大品种二次开发研究-2022C03131）