[关键词]
[摘要]
目的 建立一种同时测定注射用益气复脉(冻干)(YQFM)中4种皂苷(人参皂苷Rf、人参皂苷Rc、人参皂苷Ro和人参皂苷Rg5)含量的HPLC法。方法 用Pro Elut PLS固相萃取柱预先处理样品,然后采用Waters Symmetry C18色谱柱(250 mm×4.6 mm,5 μm),以乙腈-0.05%磷酸水溶液为流动相进行梯度洗脱,体积流量1.0 mL/min,柱温26℃,检测波长203 nm,进样量10 μL。结果 人参皂苷Rf(0.008 96~0.224 mg/mL),人参皂苷Rc(0.009 84~0.246 mg/mL),人参皂苷Ro(0.010 06~0.251 5 mg/mL),人参皂苷Rg5(0.002 61~0.026 1 mg/mL)线性良好;准确度试验(n=6)中,人参皂苷Rf、人参皂苷Rc、人参皂苷Ro和人参皂苷Rg5的平均回收率分别为93.9%、91.8%、97.4%和87.8%;20批YQFM中人参皂苷Rf、人参皂苷Rc、人参皂苷Ro和人参皂苷Rg5平均含量分别为0.233 3(0.195 2~0.271 1 mg/g)、0.438 4(0.325 8~0.497 1 mg/g)、0.739 6(0.615 8~0.910 8 mg/g)、0.066 1 mg/g(0.038 2~0.087 8 mg/g)。结论 所建立的方法简单可靠,可用于测定YQFM中人参皂苷Rf、人参皂苷Rc、人参皂苷Ro和人参皂苷Rg5 4种皂苷的含量测定。
[Key word]
[Abstract]
Objective To establish a method for simultaneous determination of four saponins (ginsenoside Rf, ginsenoside Rc ginsenoside Ro and ginsenoside Rg5) in Yiqi Fumai Lyophilized Injection (YQFM) by HPLC. Methods The samples were pretreated with the Pro Elut PLS SPE column. Waters symmetry C18 column (250 mm×4.6 mm, 5 μm) was used as the chromatographic column; acetonitrile-0.05% phosphoric acid aqueous solution was used as the mobile phase for gradient elution; the flow rate was 1.0 mL/min; the column temperature was 26℃; the detection wavelength was 203 nm; and the injection volume was 10 μL. Results The linearity of ginsenoside Rf (0.008 96-0.224 mg/mL), ginsenoside Rc (0.009 84-0.246 mg/mL), ginsenoside Ro (0.010 06-0.251 5 mg/mL), ginsenoside Rg5 (0.002 61-0.026 1 mg/mL) was good; in the accuracy test (n=6), the average recoveries of ginsenoside Rf, ginsenoside Rc, ginsenoside Ro and ginsenoside Rg5 were 93.9%, 91.8%, 97.4% and 87.8% respectively. The average contents of ginsenoside Rf, ginsenoside Rc, ginsenoside Ro and ginsenoside Rg5 for 20 batches of YQFM were 0.233 3 mg/g(0.195 2-0.271 1 mg/g), 0.438 4 mg/g (0.32 58-0.497 1 mg/g), 0.739 6 mg/g (0.615 8-0.910 8 mg/g) and 0.066 1 mg/g (0.038 2-0.087 8 mg/g), respectively. Conclusion The method is simple and reliable, and can be used for the determination of four saponins in YQFM.
[中图分类号]
R285.6
[基金项目]
天津市科技计划项目(18YFCZZC00430)