[关键词]
[摘要]
目的 探究注射用益气复脉(冻干)(YQFM)对阿霉素(DOX)所致大鼠急性心肌损伤保护作用组方合理性。方法 将140只雄性SD大鼠按体质量随机分成对照组,模型组,YQFM低、中、高剂量(0.28、0.55和1.10 g/kg)组,红参提取物低、中、高剂量(RGE)(0.21、0.42和0.85 g/kg)组,麦冬提取物(OJE)低、中、高剂量(0.30、1.27和2.55 g/kg)组和五味子提取物(SCE)的低、中、高剂量(0.31、0.63和1.27 g/kg)组。尾iv给药7 d,第5天一次性ip 25mg/kg DOX建立急性心肌损伤模型。观察大鼠状态,采集心电图,检测超声心动,腹主动脉取血,取心脏称质量;生化仪测定血清中心肌酶水平,制作心脏切片并进行苏木精-伊红(HE)染色。结果 与对照组比较,模型组大鼠精神状态差,体质量下降,心脏收缩功能异常,心电图显示T波、QT间期异常,血清中CK、LDH、AST含量均显著上升(P<0.05),且光镜观察发现心肌细胞出现病理性改变。与模型组比较,各给药组体质量均呈明显上升趋势,差异显著(P<0.05);YQFM中、高剂量,RGE、OJE高剂量和SCE中、高剂量均显著改善T波高耸(P<0.05),YQFM高剂量组起效程度最大;YQFM和RGE高剂量组显著缓解QT间期延长(P<0.05);YQFM中、高剂量组和各提取物高剂量对LVDd升高均有显著性改善(P<0.05);YQFM各剂量和各提取物中、高剂量均显著提高FS(P<0.05),各给药组均对E/A降低发挥缓解作用;YQFM各剂量均显著降低LDH水平(P<0.05),中、高剂量组显著降低CK、AST水平(P<0.05),各味药提取物高剂量显著降低CK、LDH和AST水平(P<0.05),SCE中剂量显著降低LDH和AST水平(P<0.05);YQFM中、高剂量明显改善心肌病理性变化。结论 红参、麦冬、五味子均为保护急性心肌损伤的药效组分,组合用药能够降低起效浓度,YQFM组方具有一定的科学性。
[Key word]
[Abstract]
Objective Rationality of Yiqi Fumai Lyophilized Injection against doxorubicin induced acute myocardial injury in rats. Methods 140 male SD rats were randomly divided into control group, model group, YQFM low, medium and high dose (0.28, 0.55 and 1.10 g/kg), red ginseng extract (RGE) (0.21, 0.42 and 0.85 g/kg) groups, and Ophiopogon japonicus extract (OJE) low, medium and high dose (0.30, 1.27 and 2.55 g/kg), and Schisandra chinensis extract (SCE) low, medium and high dose (0.31, 0.63 and 1.27 g/kg) groups. Treatment of the rats with relative drugs for seven days continuously and inducing acute myocardial injury by injecting DOX on the fifth day. The status of the rats was observed, electrocardiogram andechocardiography were performed, blood and heart were collected. The relevant enzyme content in the serum was detected and heart sections were stained with hematoxylin-eosin (HE). Results Compared with control group, the systolic dysfunction was abnormal in model group, the mental state of the rats was poor, the electrocardiogram T wave and QT interval were abnormal, and the serum levels of CK, LDH and AST were all increased significantly (P<0.05), and light pathological observation showed pathological changes in the control group. Compared with the model group, the body weight of each treatment group showed an upward trend, the difference was significant (P<0.05); YQFM medium and high dose, RGE, OJE high dose and SCE medium and high dose all significantly improved T wave towering (P<0.05), YQFM high dose group significantly alleviated QT interval prolongation (P<0.05); YQFM middle and high dose group and each extract high dose group had significant effect on LVDd increased (P<0.05). All YQFM doses, middle and high dose extracts of YQFM significantly increased FS (P<0.05), and each dose of YQFM significantly reduced the level of LDH (P<0.05), the levels of CK and AST in medium and high dose groups were significantly decreased (P<0.05), and the high dose of extract significantly reduced CK, LDH and AST. The levels of LDH and AST were significantly decreased by SCE medium dose (P<0.05), and the myocardial pathological changes were significantly improved by medium and high dose YQFM. Conclusion RGE, OJE and SCE are all effective components and the combination of drugs can take effects in a lower concentration works. Therefore, YQFM against myocardial injury is rational.
[中图分类号]
R965
[基金项目]
天津市科技计划项目(18YFCZZC00430、17PTSYJC00090)