在药物分析中高效液相色谱法用得非常普遍, 而色谱柱的选择直接影响实验结果与药物的质量控制。不同C18色谱柱的保留行为千差万别, 选择适宜的色谱柱对分析样品至关重要。以C18色谱柱的内部化学键合相为切入点, 介绍C18色谱柱的差异, 并综述在不同的pH环境、流动相极性、药物极性等条件下以及根据色谱柱的分类系统选择合适的键合相, 为如何正确快速的选择C18色谱柱提供参考。

HPLC is very popular in the analysis of drugs. while the column selection affects the experimental results. The retention behavior of different C18 chromatographic column differ in thousands of ways, choose the appropriate column is essential to analysis of samples. From the internal chemical bonds combination with C18 chromatographic column, introduce different C18 column. Overview how to select bonded phase in different pH environment, different mobile phase polarity,different drug polarity conditions. And according to the classification system of chromatographic column select bonded phase. Provides the reference for the C18 column selection.