After the common evaluation methods of in vitro bacteriostasis by Chinese materia medica (CMM) and the existing problems were comprehensively reviewed, we found that the evaluation criterion was not unified. The results were affected by subjective factors, there were some differences in the antibacterial effect between in vitro and in vivo, and the appropriate method was chosen without combining with the characteristics of CMM. Different bacteriostatic methods had a greater influence on the evaluation of CMM inhibition of bacteria (bacteriostasis circle, MIC), ability to kill (MBC), and the resistance of bacteria to drugs, etc. There are many research methods on the bacteriostasis experiment in vitro of CMM. Therefore, suitable methods should be chosen according to the conditions, aims, and requirements, so as to evaluate the antibacterial effect of CMM more accurately.