目的 筛选适用于分离纯化莲子心生物碱的大孔吸附树脂。方法 考察吸附性能较好的D101、AB-8、DA201 3种大孔吸附树脂对莲心碱的吸附能力及洗脱参数，并用HPLC定量分析了莲心碱的量。结果 D101树脂对莲子心中生物碱有较好的吸附分离效果。先用水洗除杂，再用6倍树脂体积、乙醇体积分数为80%、pH 3的洗脱剂洗脱，浓缩干燥，产品中莲心碱的质量分数为10.2%、产品收率为24.6%。结论 该工艺简单可行，分离效果好，适合莲子心生物碱的分离纯化。

Objective To screen the macroporous resin for the separation and purification of alkaloids from Nelumbinis Plumula. Methods The resins such as D101, AB-8, and DA201 were studied for their adsorption capability and elution parameters. The contents of liensinine were determined by HPLC. Results D101 resin had a preferable adsorptive and separative effect for the alkaloids in Nelumbinis Plumula. The preferable desorption ratio could be obtained by using 80% ethanol (pH 3) as eluting solvent and 6 BV. Under these conditions, the content of alkaloids in Nelumbinis Plumula was 10.8% and the yield was 2.6%. Conclusion The method is easy and possesses the popularization potential and good efficacy. It could be used for the separation and purification of alkaloids in Nelumbinis Plumula.

湖南省教育厅基金研究项目（11C1256）