[关键词]
[摘要]
目的:建立川续断的HPLC指纹图谱分析方法。方法:采用高效液相色谱技术,Hypersil-C18色谱柱(250 mm ×4.6 mm, 5 μm);流动相为乙睛-水,梯度洗脱;体积流量1.0 mL/min;检测波长212 nm;进样量30 μL;柱温 30 ℃。结果:建立了以 15 个共有峰为特征指纹信息的HPLC指纹图谱,发现各产地和来源的川续断的指纹图谱有一定的差异。结论:该指纹图谱方法准确可靠,重现性好,可作为川续断内在质量评价的依据。
[Key word]
[Abstract]
Objective: To establish the HPLC fingerprints of Dipsacus asperoides processed with methanol. Methods: HPLC analysis was performed on a Hypersil-C18 column (250 mm × 4. 6 mm, 5 μm) with mobile phase: acetonitrile-water under isocratic and gradient elutions and detection wavelength was 212 nm; The flow rate was 1.0 mL/min and column temperature was 30 ℃. Results: HPLC fingerprint by taking 15 common peaks as characteristic information was establised. It was found that the preparative HPLC of D. asperoides from different habitats and sources has some differences. Conclusion: The method is reliable and accurate, and can be used for the quality control of D. asperoides.
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[基金项目]
科技部国际合作项目(2008DFB30070);天津市重点项目(06TXTJJCB600)