Objective: To observe the toxic reactions and reversibility of target organ damage by alternatively continuous iv and ip injection of Ciwujia Injection (Acanthopanax Injection) in rats. Methods: The degree of toxicity was observed in rats by injection of Ciwujia Injection with hematological, blood biochemical and histopathological methods. Results: All animals survived after continuous iv and ip injection of Ciwujia Injection for 4 weeks. High-dose and low-dose group animals had no significant toxicity and no delayed toxicity occurred after treatment with various doses. Conclusion: Body weight growth rate, organ coefficient of major organs, blood cells and blood biochemical parameters had no significant effect after alternatively continuous iv and ip injection of Ciwujia Injection in rats, histopathological examination are also no toxic pathological changes, no sign of delayed toxicity was examined after three weeks of drug withdrawal, that is, Ciwujia Injection had no significant toxicity.