目的 建立高效液相色谱–串联质谱（HPLC-MS/MS）法考察芪苈强心胶囊中人参皂苷类成分（人参皂苷Rb₁、Rb₂、Rg₁、Rg₃、Rc、Rd、Re、Rf、F₂）在大鼠体内的药动学特征。方法 SD大鼠ig 1.3 g/kg芪苈强心胶囊混悬液，于给药后0.083、0.167、0.333、0.667、1、1.5、2、3、4、6、8、12、24 h经目内眦静脉丛取血，样品处理后，采用HPLC-MS/MS法测定血浆中人参皂苷Rb₁、Rb₂、Rg₁、Rg₃、Rc、Rd、Re、Rf、F₂的血药浓度。通过DAS 3.0软件拟合计算药动学参数。结果 大鼠ig芪苈强心胶囊后，血浆中人参皂苷Rb₂的药动学参数t_max为1.5 h，其余人参皂苷类成分的t_max均在0.67 h；AUC_0-∞排序依次为人参皂苷Rb₁ > 人参皂苷Rc > 人参皂苷Rg₁ > 人参皂苷Re > 人参皂苷Rb₂ > 人参皂苷Rd > 人参皂苷Rf > 人参皂苷Rg₃ > 人参皂苷F₂；t_1/2的排序依次为人参皂苷Rb₂ > 人参皂苷Rb₁ > 人参皂苷Rg₁ > 人参皂苷Re > 人参皂苷F₂ > 人参皂苷Rf > 人参皂苷Rd > 人参皂苷Rg₃ > 人参皂苷Rc。结论 该检测方法专属性强，重复性好，可用于芪苈强心胶囊中人参皂苷类成分的药动学研究。

Objective To develop a high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method to study the pharmacokinetic characteristics of ginsenoside Rb₁, Rb₂, Rg₁, Rg₃, Rc, Rd, Re, Rf, and F₂ in rat plasma after ig administration of Qili Qiangxin Capsules. Methods SD rats were given 1.3 g/kg Qili Qiangxin Capsules suspension by gavage. Blood samples were collected from inner canthus at 0.083, 0.167, 0.333, 0.667, 1, 1.5, 2, 3, 4, 6, 8, 12, and 24 h. Samples were treated, and the concentrations of ginsenoside Rb₁, Rb₂, Rg₁, Rg₃, Rc, Rd, Re, Rf, and F₂ were determined by HPLC-MS/MS. The pharmacokinetic parameters were calculated by DAS 3.0 Software. Results The pharmacokinetic parameters t_max of ginsenoside Rb₂ in rat plasma was 1.5 h, while 0.67 h for other ginsenosides. AUC_0-∞ were in the order of ginsenoside Rb₁ > Rc > Rg₁> Re > Rb₂ > Rd> Rf > Rg₃ > F₂. And t_1/2 were in the order of ginsenoside Rb₂ > Rb₁ > Rg₁ > Re > F₂ > Rf > Rd > Rg₃ > Rc. Conclusion The method is proved to be specific and repeatable, and to be suitable for determination of ginsenosides in Qili Qiangxin Capsules in rat plasma for pharmacokinetic study.

国家自然科学基金资助项目（81774227）；天津市自然科学基金资助项目（17JCZDJC34600）；天津市卫生和计划生育委员会中医中西医结合科研课题（2015050）