目的 建立高效液相色谱–荧光检测（HPLC-FLD）法测定清火片中芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚。方法 采用Agilent C₁₈色谱柱（250 mm×4.6 mm，5μm）；流动相：甲醇–0.1%磷酸溶液（78∶22）；体积流量为1 mL/min；柱温30℃；进样量为20 μL。对比HPLC-FLD法与HPLC-UV法的测定结果。结果 芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚分别在100.6～1 609.6、86.8～1 388.8、359.4～5 750.4、81.2～1 299.2、89.4～1 430.4 ng线性关系良好；游离蒽醌中芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚的平均回收率分别为93.9%、90.8%、96.2%、92.9%、89.2%，RSD值分别为2.2%、2.4%、1.0%、2.4%、0.6%；总蒽醌中各成分平均回收率分别为100.2%、85.7%、99.2%、104.5%、104.2%，RSD值分别为2.5%、2.2%、3.7%、0.5%、0.7%。结论 本方法具有重复性好、灵敏度高、专属性强、内源物干扰小等优点，与HPLC-UV法测定结果一致性较高，可用于中成药清火片的质量控制。

Objective To establish an method HPLC-FLD for the determination of aloe-emodin, rhein, emodin, chrysophanol, and physcione in Qinghuo Tablets. Methods The separation was carried out on Agilent C₁₈ chromatography column (250 mm×4.6 mm, 5 μm). The mobile phase consisted of methanol-0.1% phosphoric acid solution (78:22). The flow rate was 1 mL/min, temperature of column was set at 30℃, and volume of injection was 20 µL. The results of HPLC-FLD and HPLC-UV were compared.Results The linear ranges of aloe-emodin, rhein, emodin, chrysophanol, and physcione were 100.6-1 609.6, 86.8-1 388.8, 359.4-5 750.4, 81.2-1 299.2, and 89.4-1 430.4 ng, respectively. The average recovery rates of aloe-emodin, rhein, emodin, chrysophanol, and physcione in free anthraquinone were 93.9%, 90.8%, 96.2%, 92.9%, and 89.2%, respectively, with RSD values of 2.2%, 2.4%, 1.0%, 2.4%, and 0.6%, respectively. The average recovery rates of total anthraquinone were 100.2%, 85.7%, 99.2%, 104.5%, and 104.2%, respectively with RSD values of 2.5%, 2.2%, 3.7%, 0.5%, and 0.7%, respectively. Conclusion The method has the advantages of good repeatability, high sensitivity, strong specificity, and little interference from endogenous substances, in good agreement with HPLC-UV method, and can be used for quality control of Qinghuo Tablets.