目的 建立龙脷叶多成分定量控制方法，采用化学模式识别评价不同产地龙脷叶质量差异。方法 采用HPLC法同时测定龙脷叶中原儿茶酸、咖啡酸、6-羟基香豆素、金丝桃苷、异槲皮苷、槲皮素、山柰酚、山柰酚-3-O-龙胆二糖苷、豆甾醇、β-谷甾醇，并检测浸出物、总灰分和酸不溶性灰分。采用聚类分析（CA）、主成分分析（PCA）、因子分析（FA）和偏最小二乘回归分析（PLS-DA）法对18批不同产地的龙脷叶进行区分比较，寻找差异因子。结果 18批龙脷叶样品中各成分含量差异较大，明显分为3类，广东和广西产地的龙脷叶整体质量较好。山柰酚-3-O-龙胆二糖苷、咖啡酸、金丝桃苷、原儿茶酸、6-羟基香豆素和异槲皮苷可作为龙脷叶的质量差异标记物。结论 建立的多指标成分定量、化学模式识别可评价龙脷叶质量，为龙脷叶的质量控制和道地性研究提供参考。

Objective To establish a multi-components quantitative analysis method for Sauropi Folium, and to evaluate the quality difference of Sauropi Folium from different habitats by chemical pattern recognition methods. Methods The contents of protocatechuic acid, caffeic acid, 6-hydroxycoumarin, hyperoside, isoquercitrin, quercetin, kaempferol, kaempferol-3-O-gentionbioside, stigmasterol, and β-sitosterol in Sauropi Folium were simultaneously determined by HPLC method. Extract, total ash, and acid insoluble ash were also detected. The CA, PCA, FA, and PLS-DA were used to distinguish and compare 18 batches of Sauropi Folium from different habitats, and explore the difference factors for quality evaluation of Sauropi Folium. Results There were significant differences in the content of various components in 18 batches of Sauropi Folium. 18 Batches of Sauropi Folium were clustered into 3 categories, and the quality of Sauropi Folium from Guangdong and Guangxi Province was better. Kaempferol-3-O-gentionbioside, caffeic acid, hyperoside, protocatechuic acid, 6-hydroxycoumarin, and isoquercitrin could be used as quality difference markers for Sauropi Folium. Conclusion The established multi-index component quantitative analysis and chemical pattern recognition can evaluate the quality of Sauropi Folium, and it provides reference for quality control and genuineness research of Sauropi Folium.

R286.02

安徽省教育厅高校优秀拔尖人才培育资助项目（gxbjZD2020061）