目的 探讨香叶醇调控烷基化修复蛋白B同源物5（ALKBH5）对乳腺癌细胞上皮间质转化的影响。方法 以0、6.25、12.5、25、50、100、200 μg/mL香叶醇分别处理人乳腺癌MCF-7细胞24 h，筛选合适的香叶醇处理剂量。MCF-7细胞分为对照组、香叶醇（25、50、100 μg/mL）组、香叶醇＋空白质粒组、香叶醇＋ALKBH5组，5-乙炔基-2'脱氧尿嘧啶核苷（EdU）染色、CCK-8法检测细胞增殖；Western blotting检测细胞中ALKBH5、神经钙黏蛋白（N-cadherin）、波形蛋白（Vimentin）、E-钙黏蛋白（E-cadherin）蛋白；Transwell、划痕实验分别检测细胞侵袭、迁移；qRT-PCR检测细胞中基质金属蛋白酶（MMP）-9、迁移侵袭增强子（MIEN1）、MMP-2 mRNA表达。结果 与0 μg/mL香叶醇比较，6.25、12.5、25、50、100、200 μg/mL香叶醇作用后的MCF-7细胞活力均显著降低（P＜0.05），选取香叶醇质量浓度为25、50、100 μg/mL作为后续处理浓度。与对照组比较，香叶醇25、50、100 μg/mL组MCF-7细胞EdU阳性率、A₄₅₀值、ALKBH5、N-cadherin、Vimentin蛋白表达、细胞侵袭数、划痕愈合率及MMP-9、MIEN1、MMP-2 mRNA表达降低，E-cadherin蛋白表达升高（P＜0.05）。与香叶醇100 μg/mL组、香叶醇＋空白质粒组比较，香叶醇＋ALKBH5组MCF-7细胞EdU阳性率、A₄₅₀值、ALKBH5、N-cadherin、Vimentin蛋白表达、细胞侵袭数、划痕愈合率及MMP-9、MIEN1、MMP-2 mRNA表达升高，E-cadherin蛋白表达降低（P＜0.05）。结论 香叶醇可能通过下调ALKBH5抑制MCF-7细胞上皮间质转化，进而减弱细胞侵袭与迁移能力。

Objective To investigate the effect of geraniol on epithelial mesenchymal transition of breast cancer cells by regulating ALKBH5. Methods Human breast cancer MCF-7 cells were treated with 0, 6.25, 12.5, 25, 50, 100, 200 μg/mL geraniol for 24 hours, and the appropriate dosage of geraniol was screened. MCF-7 cells were assigned into control group, geraniol (25, 50, 100 μg/mL) group, geraniol + blank plasmid set group, and geraniol + ALKBH5 group. EdU staining and CCK-8 method were used to detect cell proliferation. Western blotting was used to detect ALKBH5, N-cadherin, Vimentin, and E-cadherin proteins in cells. Transwell and scratch experiments were used to detect cell invasion and migration, respectively. QRT-PCR was used to measure the mRNA expression of MMP-9, MIEN1, and MMP-2 in cells. Results Compared with 0 μg/mL geraniol, the activity of MCF-7 cells after treatment with 6.25, 12.5, 25, 50, 100, 200 μg/mL geraniol was significantly decreased (P < 0.05). The concentration of geraniol was 25, 50, 100 μg/mL as the subsequent treatment concentration. Compared with control group, the EdU positive rate, A₄₅₀ value, ALKBH5, N-cadherin, Vimentin protein, cell invasion number, scratch healing rate, and MMP-9, MIEN1, MMP-2 mRNA expression of MCF-7 cells in the geraniol 25, 50, 100 μg/mL groups were reduced, while E-cadherin protein were increased (P < 0.05). Compared with the geraniol 100 μg/mL group and geraniol + blank plasmid set group, the EdU positive rate, A₄₅₀ value, ALKBH5, N-cadherin, Vimentin protein, cell invasion number, scratch healing rate, and MMP-9, MIEN1, MMP-2 mRNA expression of MCF-7 cells in geraniol + ALKBH5 group were increased, while E-cadherin protein were decreased (P < 0.05). Conclusion Geraniol may inhibit epithelial mesenchymal transition in MCF-7 cells by downregulating ALKBH5, thereby weakening cell invasion and migration abilities.

R979.1

河南省医学科技攻关计划联合共建项目（LHGJ20220783，LHGJ20220784）