目的 建立高效液相色谱-一测多评法测定连花清瘟胶囊中绿原酸、甘草苷、木犀草苷、连翘酯苷A、连翘苷、大黄酸的方法。方法 使用Inertsil ODS-3色谱柱（250 mm×4.6 mm，5 μm）；流动相：乙腈-0.4%磷酸水，梯度洗脱；体积流量：1.0 mL/min；柱温：25 ℃；检测波长：280 nm。以连翘苷为内参物，建立其他5种成分的相对校正因子，一测多评法计算各成分。结果 绿原酸、甘草苷、木犀草苷、连翘酯苷A、连翘苷、大黄酸分别在0.243～4.861、0.112～2.248、0.087～1.732、0.247～4.949、0.370～7.402、0.095～0.952μg/mL线性关系良好（r≥0.999 5），平均回收率分别为99.40%、101.0%、104.5%、98.17%、99.70%、99.58%，RSD值分别为1.12%、2.53%、3.15%、2.94%、1.41%、2.84%（n＝6）。结论 该法回收率高、重复性良好，为连花清瘟胶囊建立更全面的质量控制提供了新方法。

Objective To establish an HPLC-quantitative analysis of multi-components by single marker (QAMS) to determine contents of chlorogenic acid, glycyrrhizin, luteolin, forsythiaside A, forsythin, and rhein in Lianhua Qingwen Capsules. Methods Analysis was performed on an Inertsil ODS-3 column (250 mm × 4.6 mm, 5 μm). The mobile phase consisted of acetonitrile-0.4% phosphoric acid aqueous in gradient mode. The flow rate was 1.0 mL/min, the column temperature was maintained at 25℃, and the detection wavelength was set at 280 nm. Using forsythin as the internal reference substance, the relative correction factor of the other five components was established, and the contents of each component were calculated by QAMS method. Results Chlorogenic acid, glycyrrhizin, luteolin, forsythiaside A, forsythin, and rhein all showed good linear relationship within the ranges of 0.243-4.861, 0.112-2.248, 0.087-1.732, 0.247-4.949, 0.370-7.402, and 0.095-0.952 μg/mL ( r ≥ 0.999 5), and their average recovery rates were 99.40%, 101.0%, 104.5%, 98.17%, 99.70%, and 99.58%, with the RSD of 1.12%, 2.53%, 3.15%, 2.94%, 1.41%, and 2.84%(n=6)。Conclusion This method has a high recovery rate and good repeatability, providing a new method for establishing a more comprehensive quality control of Lianhua Qingwen Capsules.

R286.02

江西省中医药管理局科技计划项目（2022A381）；江西省教育厅科学技术研究项目（GJJ2203312）