目的 建立HPLC-UV法测定金钱草中紫云英苷、山柰酚-3-O-芸香糖苷、槲皮素、山柰酚和异鼠李素。方法 采用Eclipse XDB-C₁₈色谱柱（150 mm×4.6 mm，3.5 μm），流动相为甲醇–0.4%磷酸水，梯度洗脱，检测波长360 nm，体积流量0.5 mL/min，柱温30 ℃，进样量10 μL。结果 紫云英苷、山柰酚-3-O-芸香糖苷、槲皮素、山柰酚、异鼠李素分别在22.10～176.80、21.14～169.12、17.40～174.00、51.12～408.96、23.30～186.40 μg/mL呈现良好线性关系（R²＞0.999 1）；平均加样回收率分别为99.50%、100.39%、99.93%、105.58%、98.96%，RSD值分别为1.07%、1.44%、0.57%、0.24%、0.65%（n＝6）。结论 方法的重复性好，操作简单，结果准确，可以用来控制金钱草药材及其制剂的质量。

Objective To develop an HPLC method for determination of astragalin, kaemperfol-3-O-rutinoside, quercetin, kaempferol, and isorhamnetin in Lysimachia christinae. Methods The analysis was performed on Eclipse XDB-C₁₈ column (150 mm × 4.6 mm, 3.5 μm). The mobile phase consisted of methanol and 0.4% phosphoric acid with gradient elution. The detection wavelength was 360 nm, flow rate was 0.5 mL/min, column temperature was 30 ℃, and volume of injection was 10 μL. Results Aastragalin, kaemperfol-3-O-rutinoside, quercetin, kaempferol, and isorhamnetin showed good linear relationships of 22.10 — 176.80, 21.14 — 169.12, 17.40 — 174.00, 51.12 — 408.96, and 23.30 — 186.40 μg/mL (R² ≥ 0.999 1), whose average recoveries were 99.50%, 100.39%, 99.93%, 105.58%, and 98.96% with RSD values of 1.07%, 1.44%, 0.57%, 0.24%, and 0.65% (n＝6), respectively. Conclusion The method is reproducible, simple, and accurate, which can be used for the quality control of Lysimachia christinae and its preparations.

R286.02

国家自然科学基金资助项目（81760701）