目的 考察炮制过程中茅根炭饮片UPLC指纹图谱和色度值的差异。方法 采用UPLC法建立白茅根、茅根炭的指纹图谱，并采用分光测色仪测定其色度值（L*、a*、b*），以各类分析方法分析茅根炭炮制过程中饮片指纹图谱与色度值的相关性。结果 随着炮制时间的延长，茅根炭较白茅根指纹图谱的相似度逐渐降低；峰1、5-羟甲基糠醛、峰11、4-香豆酸单位峰面积先增大后减小，其余共有峰单位峰面积均呈减小趋势；L*、b*值整体上逐渐减小，而△E*逐渐增大。5-羟甲基糠醛、峰3、峰9峰面积与色度值间具有极显著性或显著性。主成分分析共提取2个主成分，累积方差贡献率为88.870%。聚类分析结果显示，炮制时间2～6 min聚为第1类，炮制时间8～16 min聚为第2类，炮制时间18～30 min聚为第3类。正交偏最小二乘法判别分析结果显示，峰1、a*、b*、峰3、L*、峰12、E*、5-羟甲基糠醛是茅根炭饮片炮制过程中发生质量变化的主要指标。结论 不同炮制时间茅根炭饮片的色度值与UPLC指纹图谱密切相关，可为茅根炭炮制过程中监测饮片质量提供参考。

Objective To investigate the difference between UPLC fingerprint and chromaticity value of carbonized Imperatae Rhizoma of different processing time. Methods UPLC method was used to establish fingerprint, and spectrophotometric colorimeter was used to determine the chromaticity value (L*, a*, b*). And the correlation difference between UPLC fingerprint and chromaticity value of carbonized Imperatae Rhizoma during processing was analyzed using various analytical methods. Results With the prolonging of processing time, the similarity of carbonized Imperatae Rhizoma and Imperatae Rhizoma gradually decreased. The unit areas of peak 1, 5-hydroxymethylfurfural (5-HMF), peak 11, and p-coumaric acid first increased and then decreased, while the other common peaks showed a decreasing trend. The values of L* and b* gradually decreased, while △E* gradually increased. 5-HMF, peak 3, and peak 9 had extremely significant or significant correlation between peak area and chromaticity value. Two principal components were extracted by principal component analysis, and the cumulative variance contribution rate was 88.870%. The results of cluster analysis showed that the samples processed for 2 — 6, 8 — 16, and 18 — 30 min were clustered into three classes. The results of orthogonal partial least squares discriminant analysis showed that peak 1, a*, b*, peak 3, L*, peak twelve, E*, and 5-HMF were the main indexes of quality change of carbonized Imperatae Rhizoma during processing. Conclusion The chromaticity values of carbonized Imperatae Rhizoma at different processing times is closely related to UPLC fingerprint, which can provide reference for monitoring the quality of carbonized Imperatae Rhizoma.

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