目的 阐明利血平对脊髓小脑共济失调3型（SCA3）细胞模型的潜在作用。方法 通过核质分离、免疫荧光、Western blotting、微孔过滤和细胞活性等实验探索利血平对SCA3发病过程中ataxin-3核质转运、ataxin-3可溶性蛋白水平、蛋白包涵体、细胞活性等一系列病理变化的影响。结果 利血平对ataxin-3核质转运和可溶性蛋白水平没有显著性影响，但是增加了ataxin-3形成的包涵体数量，并且增加了细胞毒性。结论 利血平通过增加包涵体数量导致SCA3细胞活性减弱，为老药新用发现提供了理论依据。

Objective To clarify the potential effect of reserpine on spinocerebellar ataxia type 3 (SCA3) cell model. Methods The effects of reserpine on ataxin-3 nuclear and cytoplasmic transport, ataxin-3 soluble protein level, protein inclusion body and cell viability during the pathogenesis of SCA3 were investigated by nucleocytoplasmic separation, immunofluorescence, Western blotting, filter trap and CCK-8 methods. Results Reserpine had no significant effect on the nucleocytoplasmic transport and soluble protein level of ataxin-3, but increased the number of inclusion bodies formed by ataxin-3 and increased cytotoxicity. Conclusion Reserpine decreases the SCA3 cell viability by increasing the number of inclusion bodies, which provides a theoretical basis for the new discovery of old drugs.

R966

国家自然科学基金资助项目（82202067，32270530）；陕西省自然科学基础研究计划资助项目（2020JM-620）；西安市科技计划创新基金“文理专项”项目（2020KJWL04）；西安文理学院博士启动金（06005026）；西安文理学院院长基金（YZJJ202104）；国家级大学生创新创业项目（S202011080012，S202311080020）；省级大学生创新创业项目（S202011080058，S202111080043，S202111080031）