目的 探讨美登木素对人乳头状甲状腺癌B-CPAP细胞增殖、凋亡的影响及作用机制。方法 用不同浓度（0.19、0.76、3.05、12.21、48.83、195.31、781.25、3 125 nmol/L）的美登木素溶液处理B-CPAP细胞，SRB法检测B-CPAP细胞增殖情况。将B-CPAP细胞分为对照组及美登木素（0.1、1、10 nmol/L）组，光学显微镜观察B-CPAP细胞形态变化；流式细胞术检测B-CPAP细胞凋亡情况；Western blotting法测定B-CPAP细胞中B淋巴细胞瘤-2（Bcl-2）、半胱氨酸天冬氨酸蛋白酶-3前体（Procaspase-3）的蛋白表达量。结果 与对照组比较，美登木素能有效抑制B-CPAP细胞增殖，且呈浓度及时间相关性；随美登木素浓度增加B-CPAP细胞凋亡率逐渐升高，Bcl-2和Procaspase-3蛋白表达水平下降（P＜0.05），呈明确的浓度相关性。结论 美登木素具有抑制B-CPAP细胞增殖及诱导凋亡的作用，其机制可能与调控Bcl-2和Procaspase-3蛋白有关。

Objective To investigate the effects of maytansine on the proliferation and apoptosis of B-CPAP cells and its mechanism. Methods B-CPAP cells were treated with different concentrations (0.19, 0.76, 3.05, 12.21, 48.83, 195.31, 781.25, and 3 125 nmol/L) of maytansine solution. The proliferation of B-CPAP cells was detected by SRB. B-CPAP cells were divided into control group and maytansine (0.1, 1, and 10 nmol/L) group. The change of cellular morphology was observed by invert microscope, and the flow cytometry was used to detect the cell apoptosis rate and the expression of Bcl-2 and Procaspase-3 protein was assayed by Western blotting. Results Compared with the control group, maytansine could proliferation inhibited B-CPAP cell in concentration and time-dependent manner. The apoptosis rate of B-CPAP cells gradually increased, and the expression levels of Bcl-2 and Procaspase-3 proteins decreased (P < 0.05) with concentration dependent. Conclusion Maytansine could inhibit the proliferation and induce apoptosis of B-CPAP cells, and its mechanism may be related to the regulation of Bcl-2 and Procaspase-3 proteins.

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沧州市重点研发计划项目（204106038）；天津市第二批卫生健康行业高层次人才选拔培养工程（TJSJMYXYC-D2-034）；天津市卫生健康委天津市中医药管理局中医中西医结合科研课题（2021072）