目的 调查分析南方医科大学附属深圳妇幼保健院分离真菌的分布特点和药物敏感性情况,为妇幼真菌感染疾病的临床诊治、耐药性监测和流行病学研究提供参考。方法 回顾分析南方医科大学附属深圳妇幼保健院2018年1月-2022年12月标本采集、真菌鉴定和抗真菌药敏试验的结果数据。结果 共分离真菌3 350株,前2位分别为白色念珠菌1 542株、光滑念珠菌223株。阳性标本以阴道分泌物/宫颈分泌物为主。妇科和产科分离到的真菌最多。白色念珠菌对伊曲康唑、伏立康唑的5年耐药率分别为9.58%、4.12%,对两性霉素B和5-氟胞嘧啶的5年敏感率分别为99.79%、98.01%,对氟康唑的历年敏感率有逐渐升高的态势。光滑念珠菌、近平滑念珠菌和热带念珠菌对两性霉素B及5-氟胞嘧啶的敏感率均为100.00%。克柔念珠菌对两性霉素B和伏立康唑100.00%敏感。结论 南方医科大学附属深圳妇幼保健院分离真菌以念珠菌属为主,其中白色念珠菌最多。阴道分泌物/宫颈分泌物是主要真菌阳性分离标本。白色念珠菌对两性霉素B的耐药率最低、敏感率最高,对伊曲康唑的耐药率最高、敏感率最低。光滑念珠菌、近平滑念珠菌、热带念珠菌和克柔念珠菌对两性霉素B和5-氟胞嘧啶均无耐药性。光滑念珠菌对伊曲康唑耐药率最高、敏感率最低。

Objective To investigate and analyze the distribution characteristics and drug susceptibility of fungi isolated from Shenzhen Maternity and Child Healthcare Hospital, Southern Medical University, and to provide reference for clinical diagnosis and treatment of maternal and child fungal infectious diseases, antifungal resistance surveillance and epidemiological research. Methods The resulting data of specimen collection, fungus identification and antifungal susceptibility testing in Shenzhen Maternity and Child Healthcare Hospital, Southern Medical University, from January 2018 to December 2022 were collected for descriptive retrospective analysis. Results 3 350 Strains of non-repetitive fungi were isolated. The top two were 1 542 strains of Candida albicans and 223 strains of Candida glabrata, respectively. The fungal positive specimens were mainly vaginal secretions/cervical secretions. Among the permanent departments, the most fungi were isolated from gynaecology and obstetrics department. The five-year drug resistance rates of Candida albicans to itraconazole and voriconazole were 9.58% and 4.12%, respectively, the five-year susceptibility rates to amphotericin B and 5-fluorocytosine were 99.79% and 98.01%, respectively, and the susceptibility rate to fluconazole over the years was gradually increasing. The susceptibility rates of Candida glabrata, Candida parapsilosis, and Candida tropicalis to amphotericin B and 5-fluorocytosine were all 100.00%. Candida krusei was 100.00% sensitive to amphotericin B and voriconazole. Conclusions From 2018 to 2022, the main fungi isolated from Shenzhen Maternal and Child Health Hospital affiliated to Southern Medical University were Candida, among which Candida albicans was the most common. Vaginal secretion/cervical secretion is the main fungal positive isolation specimen. Candida albicans had the lowest resistance rate and the highest sensitivity rate to amphotericin B, and the highest resistance rate and the lowest sensitivity rate to itraconazole. Candida glabrata, Candida parapsilosis, and Candida tropicalis, and Candida krusei have no resistance to amphotericin B and 5-fluorocytosine. Candida glabrata has the highest resistance rate to itraconazole and the lowest sensitivity rate.

R978.5

国家自然科学基金资助项目(81702087)；广东省基础与应用基础研究基金项目(2020A1515010590)；广东省医学科学技术研究基金项目(A2020097)；深圳市科技计划项目(JCYJ20220530155213031)